Influence of hyperthyroidism on the effect of adenosine transport blockade assessed by a novel method in guinea pig atria

Denes Karsai, R. Gesztelyi, Judit Zsuga, Anita Jakab, Levente Szendrei, B. Juhász, I. Bak, Gergo Szabo, I. Lekli, M. Vecsernyés, E. Varga, Andras Jozsef Szentmiklosi, A. Tósaki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The aim of the present study was to investigate the effect of hyperthyroidism on the trans-sarcolemmal adenosine (Ado) flux via equilibrative and nitrobenzylthioinosine (NBTI)-sensitive nucleoside transporters (ENT1) in guinea pig atria, by assessing the change in the Ado concentration of the interstitial fluid ([Ado]ISF) under nucleoside transport blockade with NBTI. For the assessment, we applied our novel method, which estimates the change in [Ado]ISF utilizing the altered inotropic response to N6-cyclopentyladenosine (CPA), a relative stable selective agonist of A1 Ado receptors, by providing a relative index, the equivalent concentration of CPA. Our results show an interstitial Ado accumulation upon ENT1 blockade, which was more extensive in the hyperthyroid samples (CPA concentrations equieffective with the surplus [Ado]ISF were two to three times higher in hyperthyroid atria than in euthyroid ones, with regard to the negative inotropic effect of CPA and Ado). This suggests an enhanced Ado influx via ENT1 in hyperthyroid atria. It is concluded that hyperthyroidism does not alter the prevailing direction of the Ado transport, moreover intensifies the Ado influx in the guinea pig atrium.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalCell Biochemistry and Biophysics
Volume47
Issue number1
Publication statusPublished - Jan 2007

Fingerprint

Hyperthyroidism
Adenosine
Guinea Pigs
Adenosine A1 Receptor Agonists
Nucleoside Transport Proteins
Extracellular Fluid
Nucleosides
Fluxes
Fluids

Keywords

  • A1 adenosine receptors
  • Atria
  • Guinea pig
  • Heart
  • Interstitial adenosine
  • Receptorial responsiveness method
  • Thyroid hormones

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Biophysics

Cite this

Influence of hyperthyroidism on the effect of adenosine transport blockade assessed by a novel method in guinea pig atria. / Karsai, Denes; Gesztelyi, R.; Zsuga, Judit; Jakab, Anita; Szendrei, Levente; Juhász, B.; Bak, I.; Szabo, Gergo; Lekli, I.; Vecsernyés, M.; Varga, E.; Szentmiklosi, Andras Jozsef; Tósaki, A.

In: Cell Biochemistry and Biophysics, Vol. 47, No. 1, 01.2007, p. 45-52.

Research output: Contribution to journalArticle

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