Influence of Fe concentration in the medium on multicellular pollen grains and haploid plants induced by mannitol pretreatment in barley (Hordeum vulgare L.)

A. Pulido, F. Bakos, A. Castillo, M. P. Vallés, B. Barnabás, A. Olmedilla

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study aims to clarify the short- and long-term effects of the iron concentration in the medium on androgenesis induced in barley by isolated microspore culture. The ultrastructural features and pectin composition of the intine wall were studied in the initial stages of androgenesis. The evolution of electron-dense iron deposits on the intine was analysed in multicellular pollen grains obtained by isolated microspore culture performed for 3, 6, and 9 days using various concentrations of FeNa2 EDTA. Finally, the number of embryo-like structures and green plants obtained by microspore culture using different Fe concentrations was evaluated in order to estimate the optimum concentration for isolated microspore culture.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalProtoplasma
Volume228
Issue number1-3
DOIs
Publication statusPublished - Aug 1 2006

Keywords

  • Androgenesis
  • Culture medium
  • Hordeum vulgare L.
  • Iron
  • Microspore culture
  • Pectin

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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