Influence of diabetic state and that of different sulfonylureas on the size of myocardial infarction with and without ischemic preconditioning in rabbits

Eve Nieszner, I. Posa, E. Kocsis, G. Pogátsa, I. Préda, M. Z. Koltai

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The sensitivity of the myocardium to ischemia and the level of protection achieved by ischemic preconditioning is shaped by the joint influence of several mechanisms in diabetes mellitus. In vivo studies were made in alloxan diabetic and non-diabetic control rabbits to assess if the effects of preconditioning and sulfonylurea pretreatment with either glibenclamide or glimepiride (0.05-0.2-0.6 μmol kg-1) influence the extent of the infarcted area caused by one hour ligature of the left coronary artery. For our study, we defined preconditioning as 2 minutes of ischemia followed by 2 minutes of reperfusion, which was repeated 3 times. The interrelationship of the diabetic pathophysiological state, and sulfonylurea treatment during ischemic preconditioning were studied by comparing the infarcted areas and the rate of infarction to risk areas in left ventricular slices using computer planimetry. In healthy control rabbits preconditioning reduced infarcted area (29.6±3.0% vs. 48.8±2.8% pGlib-0.05: 0.69±0.06 p<0.004 vs. HPGlib-0.2: 0.72±0.09 P<0.002 vs. HPGlib-0.6: 0.75± 0.04 pGlib-0.6: 0.77±0.17 vs. DPGlib-0.05: 0.55 <0.03 p <0.047) and the consequences of the diabetic state blocked the salutary effect. Glimepiride had no considerable influence on the protective effect, either in control nor in diabetic animals. Glibenclamide and glimepiride, presumably due to their different sulfonylurea receptor affinity in the heart, resulted in different influence on preconditioning in healthy control animals. Glibenclamide treatment seemed to be more harmful when less K+ATP channels were activated. The accomplishment of myocardial preconditioning in diabetes mellitus is claimed to be determined by the interaction of both metabolically influenced K+ATP channel activity and the dose of sulfonylurea.

Original languageEnglish
Pages (from-to)212-218
Number of pages7
JournalExperimental and Clinical Endocrinology and Diabetes
Volume110
Issue number5
DOIs
Publication statusPublished - 2002

Fingerprint

glimepiride
Ischemic Preconditioning
Glyburide
Myocardial Infarction
Rabbits
Diabetes Mellitus
Myocardial Ischemic Preconditioning
Ischemia
Adenosine Triphosphate
Sulfonylurea Receptors
Alloxan
Infarction
Reperfusion
Ligation
Coronary Vessels
Myocardium
Joints
Therapeutics

Keywords

  • Carbohydrate metabolism
  • Preconditioning
  • Sulfonylurea

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

@article{4fcf6b1b37a84e918ba002af3a2bd72c,
title = "Influence of diabetic state and that of different sulfonylureas on the size of myocardial infarction with and without ischemic preconditioning in rabbits",
abstract = "The sensitivity of the myocardium to ischemia and the level of protection achieved by ischemic preconditioning is shaped by the joint influence of several mechanisms in diabetes mellitus. In vivo studies were made in alloxan diabetic and non-diabetic control rabbits to assess if the effects of preconditioning and sulfonylurea pretreatment with either glibenclamide or glimepiride (0.05-0.2-0.6 μmol kg-1) influence the extent of the infarcted area caused by one hour ligature of the left coronary artery. For our study, we defined preconditioning as 2 minutes of ischemia followed by 2 minutes of reperfusion, which was repeated 3 times. The interrelationship of the diabetic pathophysiological state, and sulfonylurea treatment during ischemic preconditioning were studied by comparing the infarcted areas and the rate of infarction to risk areas in left ventricular slices using computer planimetry. In healthy control rabbits preconditioning reduced infarcted area (29.6±3.0{\%} vs. 48.8±2.8{\%} pGlib-0.05: 0.69±0.06 p<0.004 vs. HPGlib-0.2: 0.72±0.09 P<0.002 vs. HPGlib-0.6: 0.75± 0.04 pGlib-0.6: 0.77±0.17 vs. DPGlib-0.05: 0.55 <0.03 p <0.047) and the consequences of the diabetic state blocked the salutary effect. Glimepiride had no considerable influence on the protective effect, either in control nor in diabetic animals. Glibenclamide and glimepiride, presumably due to their different sulfonylurea receptor affinity in the heart, resulted in different influence on preconditioning in healthy control animals. Glibenclamide treatment seemed to be more harmful when less K+ATP channels were activated. The accomplishment of myocardial preconditioning in diabetes mellitus is claimed to be determined by the interaction of both metabolically influenced K+ATP channel activity and the dose of sulfonylurea.",
keywords = "Carbohydrate metabolism, Preconditioning, Sulfonylurea",
author = "Eve Nieszner and I. Posa and E. Kocsis and G. Pog{\'a}tsa and I. Pr{\'e}da and Koltai, {M. Z.}",
year = "2002",
doi = "10.1055/s-2002-33069",
language = "English",
volume = "110",
pages = "212--218",
journal = "Experimental and Clinical Endocrinology and Diabetes",
issn = "0947-7349",
publisher = "Thieme",
number = "5",

}

TY - JOUR

T1 - Influence of diabetic state and that of different sulfonylureas on the size of myocardial infarction with and without ischemic preconditioning in rabbits

AU - Nieszner, Eve

AU - Posa, I.

AU - Kocsis, E.

AU - Pogátsa, G.

AU - Préda, I.

AU - Koltai, M. Z.

PY - 2002

Y1 - 2002

N2 - The sensitivity of the myocardium to ischemia and the level of protection achieved by ischemic preconditioning is shaped by the joint influence of several mechanisms in diabetes mellitus. In vivo studies were made in alloxan diabetic and non-diabetic control rabbits to assess if the effects of preconditioning and sulfonylurea pretreatment with either glibenclamide or glimepiride (0.05-0.2-0.6 μmol kg-1) influence the extent of the infarcted area caused by one hour ligature of the left coronary artery. For our study, we defined preconditioning as 2 minutes of ischemia followed by 2 minutes of reperfusion, which was repeated 3 times. The interrelationship of the diabetic pathophysiological state, and sulfonylurea treatment during ischemic preconditioning were studied by comparing the infarcted areas and the rate of infarction to risk areas in left ventricular slices using computer planimetry. In healthy control rabbits preconditioning reduced infarcted area (29.6±3.0% vs. 48.8±2.8% pGlib-0.05: 0.69±0.06 p<0.004 vs. HPGlib-0.2: 0.72±0.09 P<0.002 vs. HPGlib-0.6: 0.75± 0.04 pGlib-0.6: 0.77±0.17 vs. DPGlib-0.05: 0.55 <0.03 p <0.047) and the consequences of the diabetic state blocked the salutary effect. Glimepiride had no considerable influence on the protective effect, either in control nor in diabetic animals. Glibenclamide and glimepiride, presumably due to their different sulfonylurea receptor affinity in the heart, resulted in different influence on preconditioning in healthy control animals. Glibenclamide treatment seemed to be more harmful when less K+ATP channels were activated. The accomplishment of myocardial preconditioning in diabetes mellitus is claimed to be determined by the interaction of both metabolically influenced K+ATP channel activity and the dose of sulfonylurea.

AB - The sensitivity of the myocardium to ischemia and the level of protection achieved by ischemic preconditioning is shaped by the joint influence of several mechanisms in diabetes mellitus. In vivo studies were made in alloxan diabetic and non-diabetic control rabbits to assess if the effects of preconditioning and sulfonylurea pretreatment with either glibenclamide or glimepiride (0.05-0.2-0.6 μmol kg-1) influence the extent of the infarcted area caused by one hour ligature of the left coronary artery. For our study, we defined preconditioning as 2 minutes of ischemia followed by 2 minutes of reperfusion, which was repeated 3 times. The interrelationship of the diabetic pathophysiological state, and sulfonylurea treatment during ischemic preconditioning were studied by comparing the infarcted areas and the rate of infarction to risk areas in left ventricular slices using computer planimetry. In healthy control rabbits preconditioning reduced infarcted area (29.6±3.0% vs. 48.8±2.8% pGlib-0.05: 0.69±0.06 p<0.004 vs. HPGlib-0.2: 0.72±0.09 P<0.002 vs. HPGlib-0.6: 0.75± 0.04 pGlib-0.6: 0.77±0.17 vs. DPGlib-0.05: 0.55 <0.03 p <0.047) and the consequences of the diabetic state blocked the salutary effect. Glimepiride had no considerable influence on the protective effect, either in control nor in diabetic animals. Glibenclamide and glimepiride, presumably due to their different sulfonylurea receptor affinity in the heart, resulted in different influence on preconditioning in healthy control animals. Glibenclamide treatment seemed to be more harmful when less K+ATP channels were activated. The accomplishment of myocardial preconditioning in diabetes mellitus is claimed to be determined by the interaction of both metabolically influenced K+ATP channel activity and the dose of sulfonylurea.

KW - Carbohydrate metabolism

KW - Preconditioning

KW - Sulfonylurea

UR - http://www.scopus.com/inward/record.url?scp=0036024167&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036024167&partnerID=8YFLogxK

U2 - 10.1055/s-2002-33069

DO - 10.1055/s-2002-33069

M3 - Article

VL - 110

SP - 212

EP - 218

JO - Experimental and Clinical Endocrinology and Diabetes

JF - Experimental and Clinical Endocrinology and Diabetes

SN - 0947-7349

IS - 5

ER -