Influence of ceramide on the internal structure and hydration of the phospholipid bilayer studied by neutron and X-ray scattering

M. A. Kiselev, E. V. Zemlyanaya, N. Y. Ryabova, T. Hauss, L. Almásy, S. S. Funari, J. Zbytovska, D. Lombardo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Small angle neutron scattering (SANS), neutron diffraction and X-ray powder diffraction were used to investigate influence of N-stearoyl phytosphingosine (CER[NP]) and α-hydroxy-N-stearoyl phytosphingosine (CER[AP]) on the internal structure and hydration of DMPC membrane in fully and partly hydrated states at T = 30°C. Application of Fourier analysis for diffraction data and model calculations for the SANS data evidence that addition of both CER[NP] and CER[AP] in small concentrations promotes significant changes in the organization of DMPC bilayers, such as the increase of the hydrophobic core region. SANS data evidence a decrease in the average radius and polydispersity of the vesicles that can be ascribed to hydrogen bonds interactions that favor tight lipid packing with a compact, more rigid character.

Original languageEnglish
Pages (from-to)319-325
Number of pages7
JournalApplied Physics A: Materials Science and Processing
Volume116
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

phytosphingosine
Ceramides
Phospholipids
Neutron scattering
X ray scattering
Hydration
Dimyristoylphosphatidylcholine
hydration
Neutrons
neutron scattering
neutrons
scattering
x rays
Fourier analysis
Polydispersity
Neutron diffraction
diffraction
X ray powder diffraction
Lipids
neutron diffraction

ASJC Scopus subject areas

  • Materials Science(all)
  • Chemistry(all)

Cite this

Influence of ceramide on the internal structure and hydration of the phospholipid bilayer studied by neutron and X-ray scattering. / Kiselev, M. A.; Zemlyanaya, E. V.; Ryabova, N. Y.; Hauss, T.; Almásy, L.; Funari, S. S.; Zbytovska, J.; Lombardo, D.

In: Applied Physics A: Materials Science and Processing, Vol. 116, No. 1, 2014, p. 319-325.

Research output: Contribution to journalArticle

Kiselev, M. A. ; Zemlyanaya, E. V. ; Ryabova, N. Y. ; Hauss, T. ; Almásy, L. ; Funari, S. S. ; Zbytovska, J. ; Lombardo, D. / Influence of ceramide on the internal structure and hydration of the phospholipid bilayer studied by neutron and X-ray scattering. In: Applied Physics A: Materials Science and Processing. 2014 ; Vol. 116, No. 1. pp. 319-325.
@article{90ccd30fffc44fb79ef9db6c6eb3fd6e,
title = "Influence of ceramide on the internal structure and hydration of the phospholipid bilayer studied by neutron and X-ray scattering",
abstract = "Small angle neutron scattering (SANS), neutron diffraction and X-ray powder diffraction were used to investigate influence of N-stearoyl phytosphingosine (CER[NP]) and α-hydroxy-N-stearoyl phytosphingosine (CER[AP]) on the internal structure and hydration of DMPC membrane in fully and partly hydrated states at T = 30°C. Application of Fourier analysis for diffraction data and model calculations for the SANS data evidence that addition of both CER[NP] and CER[AP] in small concentrations promotes significant changes in the organization of DMPC bilayers, such as the increase of the hydrophobic core region. SANS data evidence a decrease in the average radius and polydispersity of the vesicles that can be ascribed to hydrogen bonds interactions that favor tight lipid packing with a compact, more rigid character.",
author = "Kiselev, {M. A.} and Zemlyanaya, {E. V.} and Ryabova, {N. Y.} and T. Hauss and L. Alm{\'a}sy and Funari, {S. S.} and J. Zbytovska and D. Lombardo",
year = "2014",
doi = "10.1007/s00339-013-8123-3",
language = "English",
volume = "116",
pages = "319--325",
journal = "Applied Physics",
issn = "0340-3793",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Influence of ceramide on the internal structure and hydration of the phospholipid bilayer studied by neutron and X-ray scattering

AU - Kiselev, M. A.

AU - Zemlyanaya, E. V.

AU - Ryabova, N. Y.

AU - Hauss, T.

AU - Almásy, L.

AU - Funari, S. S.

AU - Zbytovska, J.

AU - Lombardo, D.

PY - 2014

Y1 - 2014

N2 - Small angle neutron scattering (SANS), neutron diffraction and X-ray powder diffraction were used to investigate influence of N-stearoyl phytosphingosine (CER[NP]) and α-hydroxy-N-stearoyl phytosphingosine (CER[AP]) on the internal structure and hydration of DMPC membrane in fully and partly hydrated states at T = 30°C. Application of Fourier analysis for diffraction data and model calculations for the SANS data evidence that addition of both CER[NP] and CER[AP] in small concentrations promotes significant changes in the organization of DMPC bilayers, such as the increase of the hydrophobic core region. SANS data evidence a decrease in the average radius and polydispersity of the vesicles that can be ascribed to hydrogen bonds interactions that favor tight lipid packing with a compact, more rigid character.

AB - Small angle neutron scattering (SANS), neutron diffraction and X-ray powder diffraction were used to investigate influence of N-stearoyl phytosphingosine (CER[NP]) and α-hydroxy-N-stearoyl phytosphingosine (CER[AP]) on the internal structure and hydration of DMPC membrane in fully and partly hydrated states at T = 30°C. Application of Fourier analysis for diffraction data and model calculations for the SANS data evidence that addition of both CER[NP] and CER[AP] in small concentrations promotes significant changes in the organization of DMPC bilayers, such as the increase of the hydrophobic core region. SANS data evidence a decrease in the average radius and polydispersity of the vesicles that can be ascribed to hydrogen bonds interactions that favor tight lipid packing with a compact, more rigid character.

UR - http://www.scopus.com/inward/record.url?scp=84903312435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903312435&partnerID=8YFLogxK

U2 - 10.1007/s00339-013-8123-3

DO - 10.1007/s00339-013-8123-3

M3 - Article

AN - SCOPUS:84903312435

VL - 116

SP - 319

EP - 325

JO - Applied Physics

JF - Applied Physics

SN - 0340-3793

IS - 1

ER -