Influence of antithymocyte globulin treatment of brain-dead organ donor on inflammatory response in cardiac grafts: an experimental study in mice

Jamila Kremer, Gabriela K. Muschitz, Klaus Aumayr, Philipp Moser, G. Szabó, Alexander Weymann, Andreas Zuckermann, Bruno K. Podesser

Research output: Contribution to journalArticle

Abstract

The expression of proinflammatory cytokines in donor hearts after antithymocyte globulin (ATG) treatment given prior to organ removal was evaluated to analyze changes in inflammatory response. Adult female OF-1 mice were randomized into brain death (BD) groups (BD Control, BD ATG) with or without treatment, and Controls (Control, ATG). BD induction was performed through gradual inflation of an intracranial positioned balloon catheter. At the end of a 6-h observation period, ATG (1 mg/kg BW) was given intravenously. After 45 min, the donor hearts were removed. Proinflammatory markers IL-2 and IL-6 were examined using ELISA and immunohistochemistry staining. After single administration of ATG, the inflammatory reaction in the myocardium showed a significant reduction in IL-2 expression (BD Control vs. BD ATG, P = 0.033). Our investigation showed expected increase in proinflammatory mediators after BD. This increase was abolished by single infusion of ATG, indicated by significant reduction in IL-2 levels in the myocardium. We observed a reduction of IL-6 deposition in media cells in ATG-treated specimens. Further research is necessary to evaluate the role of ATG in donor management considering a potentially positive effect of ATG on IL-2-directed inflammatory response and possible reduction of IL-6-mediated vascular changes.

Original languageEnglish
Pages (from-to)1329-1336
Number of pages8
JournalTransplant International
Volume29
Issue number12
DOIs
Publication statusPublished - Dec 1 2016

Fingerprint

Brain Death
Antilymphocyte Serum
Tissue Donors
Transplants
Interleukin-2
Therapeutics
Interleukin-6
Myocardium
Economic Inflation
Blood Vessels
Catheters
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Observation
Staining and Labeling
Cytokines

Keywords

  • antithymocyte globulin
  • brain death
  • inflammation
  • organ quality
  • transplantation

ASJC Scopus subject areas

  • Transplantation

Cite this

Influence of antithymocyte globulin treatment of brain-dead organ donor on inflammatory response in cardiac grafts : an experimental study in mice. / Kremer, Jamila; Muschitz, Gabriela K.; Aumayr, Klaus; Moser, Philipp; Szabó, G.; Weymann, Alexander; Zuckermann, Andreas; Podesser, Bruno K.

In: Transplant International, Vol. 29, No. 12, 01.12.2016, p. 1329-1336.

Research output: Contribution to journalArticle

Kremer, Jamila ; Muschitz, Gabriela K. ; Aumayr, Klaus ; Moser, Philipp ; Szabó, G. ; Weymann, Alexander ; Zuckermann, Andreas ; Podesser, Bruno K. / Influence of antithymocyte globulin treatment of brain-dead organ donor on inflammatory response in cardiac grafts : an experimental study in mice. In: Transplant International. 2016 ; Vol. 29, No. 12. pp. 1329-1336.
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