Influence of aminoglycoside antibiotics on the thermal behaviour and structural features of DPPE-DPPG model membranes

Ágnes Oszlánczi, Attila Bóta, Erwin Klumpp

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effects of three aminoglycosides (AGs), tobramycin, streptomycin and spectinomycin on 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE)-1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-1-glycerol] (DPPG)/water systems with 0.05 and 0.2 DPPG/DPPE + DPPG molar fractions were examined by using differential scanning calorimetry (DSC), simultaneous small- and wide-angle X-ray scattering (SWAXS), and freeze-fracture transmission electronmicroscopy (FF-TE) at 10-1 AG/lipid molar ratio in the doped systems. The effects induced by the AGs strongly depend on DPPG/DPPE + DPPG molar fraction. In the presence of the AGs regular lyotropic structures form as opposed to the complex and badly correlated layer structures of the pure 0.2 DPPG/DPPE + DPPG molar fraction system. The investigated AGs possess different structural and thermotropic effects: tobramycin and streptomycin which are true aminoglycosides decrease the main transition enthalpy in both model systems and induce the formation of correlated layer arrangements, while the occurrence of spectinomycin, a non-aminoglycoside aminocyclitol still ranked as an AG, results in a hexagonal phase as it can be deduced from the SAXS patterns and visually observed in the electronmicrographs.

Original languageEnglish
Pages (from-to)141-148
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume75
Issue number1
DOIs
Publication statusPublished - Jan 1 2010

Keywords

  • Aminoglycosides
  • DPPE-DPPG liposomes
  • DSC
  • Freeze-fracture
  • Small- and wide-angle X-ray scattering (SWAXS)

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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