Inflammatory- and immune responses in relation to bacterial replication in mice following re-infections with Chlamydophila pneumoniae

Z. Kis, K. Burian, B. Treso, K. Acs, Z. Prohaszka, G. Fust, E. Gonczol, V. Endresz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: Investigation of chronic infections with Chlamydophila pneumoniae. Methods: BALB/c mice were repeatedly infected with C. pneumoniae and tested during a 1-year period. Production of histamine, IFN-γ, IL-6 and antibodies was monitored by ELISA. Live bacteria were cultured and DNA was detected by PCR. Cellular immunity was tested by ELISPOT. Results: After re-infections, culture positivity and persistence of DNA in lungs and blood were shorter. Detection of DNA at late time points indicated persistent infection in a few mice. Histamine was produced after primary and re-infections, and the level correlated with the number of viable bacteria in lung. IFN-γ, IL-6 levels, I2/I1 ratio, IgA titres, and level of chlamydial heat-shock protein antibodies were higher after re-infections. IgM antibodies were demonstrated even after re-infections. High number of IFN-γ-producing splenocytes was observed after the third inoculation. Conclusion: These results promote an understanding of the patho- and immune mechanisms after C. pneumoniae re-infections.

Original languageEnglish
Pages (from-to)287-295
Number of pages9
JournalInflammation Research
Volume57
Issue number6
DOIs
Publication statusPublished - Jun 1 2008

Keywords

  • C. Pneumoniae
  • Histamine
  • Immune response
  • Re-infections

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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