Induction of wheat/barley translocations by irradiation and their detection using fluorescence in situ hybridization

É Szakács, K. Kruppa, I. Molnár, M. Molnár-Láng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The aim of the present study was to test the efficiency of gamma irradiation in inducing translocations between wheat and barley genomes using addition lines. The Martonvásári 9 kr1-Igri disomic addition set, previously produced in Martonvásár, was irradiated with gamma rays. The pattern of irradiation-induced intergenomic chromosome rearrangements was analysed in the mutagenized (M0) generation by genomic in situ hybridization (GISH). Centric fusions and a wide variety of reciprocal, terminal and interstitial translocations were frequently induced. The intergeneric translocations produced here are expected to be stabilized in later backcross progenies as a set of introgression lines carrying few but distinct rearrangements.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalActa Agronomica Hungarica
Volume58
Issue number3
DOIs
Publication statusPublished - Sep 1 2010

Fingerprint

fluorescence in situ hybridization
gamma radiation
irradiation
barley
disomics
wheat
introgression
in situ hybridization
chromosomes
genomics
genome
testing

Keywords

  • gamma irradiation
  • genetic stability
  • Hordeum vulgare
  • in situ hybridization
  • translocations
  • Triticum aestivum
  • winter wheat-winter barley addition lines

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Induction of wheat/barley translocations by irradiation and their detection using fluorescence in situ hybridization. / Szakács, É; Kruppa, K.; Molnár, I.; Molnár-Láng, M.

In: Acta Agronomica Hungarica, Vol. 58, No. 3, 01.09.2010, p. 203-209.

Research output: Contribution to journalArticle

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