Induction of systemic and mucosa[ immune responses in cotton rats immunized with human adenovirus type 5 recombinants expressing the full and truncated forms of bovine herpesvirus type 1 glycoprotein gD

Suresh K. Mittal, Zsuzsanna Papp, Suresh K. Tikoo, Maria E. Baca-Estrada, Dongwan Yoo, M. Benkó, Lorne A. Babiuk

Research output: Contribution to journalArticle

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Abstract

We generated both replication-incompetent (HAd5-gD-E1 and HAd5-tgD-E1) and replication-competent (HAd5-gD-E3 and HAd5-tgD-E3) human adenovirus type 5 (HAd5) recombinants expressing the full (gD) or truncated form (tgD) of the glycoprotein gD gene of bovine herpesvirus type 1 (BHV-1). Recombinant gD and tgD expressed by HAd5-gD-E1 and HAd5-gD-E3 and by HAd5-tgD-E1 and HAd5-tgD-E3, respectively, were recognized by gD-specific monoclonal antibodies (MAbs) directed against linear and conformational epitopes, suggesting that antigenicity of recombinant gD and tgD was similar to that of the native gD expressed in BHV-1 infected cells. In HAd5-gD-E1- or HAd5-gD-E3-inoculated cotton rats there was a strong gD- and HAd5-specific IgG and IgA antibody response. The immune response was significantly lower in animals similarly immunized with HAd5-tgD-E1 or HAd5-tgD-E3, indicating that live adenovirus vaccine vectors may be better suited to the full-length form of glycoprotein gD than its truncated form. After a BHV-1 challenge, no infectious BHV-1 virions were isolated from the trachea of cotton rats previously immunized with HAd5-gD-E1 or HAd5-gD-E3. These results suggest that adenovirus E1 insertion (replication-incompetent) and E3 insertion (replication-competent) vectors have excellent potential for use in developing live recombinant virus vaccines and provide evidence that the cotton rat model can be used in BHV-1 vaccination-challenge trials.

Original languageEnglish
Pages (from-to)299-309
Number of pages11
JournalVirology
Volume222
Issue number2
DOIs
Publication statusPublished - Aug 15 1996

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Sigmodontinae
Human Adenoviruses
Mucous Membrane
Bovine Herpesvirus 1
bovine herpesvirus type-1 glycoproteins
Glycoproteins
Adenovirus Vaccines
Synthetic Vaccines

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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Induction of systemic and mucosa[ immune responses in cotton rats immunized with human adenovirus type 5 recombinants expressing the full and truncated forms of bovine herpesvirus type 1 glycoprotein gD. / Mittal, Suresh K.; Papp, Zsuzsanna; Tikoo, Suresh K.; Baca-Estrada, Maria E.; Yoo, Dongwan; Benkó, M.; Babiuk, Lorne A.

In: Virology, Vol. 222, No. 2, 15.08.1996, p. 299-309.

Research output: Contribution to journalArticle

Mittal, Suresh K. ; Papp, Zsuzsanna ; Tikoo, Suresh K. ; Baca-Estrada, Maria E. ; Yoo, Dongwan ; Benkó, M. ; Babiuk, Lorne A. / Induction of systemic and mucosa[ immune responses in cotton rats immunized with human adenovirus type 5 recombinants expressing the full and truncated forms of bovine herpesvirus type 1 glycoprotein gD. In: Virology. 1996 ; Vol. 222, No. 2. pp. 299-309.
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AU - Baca-Estrada, Maria E.

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AU - Benkó, M.

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