Induction of resistance with benzothiadiazole in sunflower: A comparison of biotrophic vs. necrotrophic pathosystems

K. Körösi, F. Virányi, B. Barna, R. Bán

Research output: Contribution to journalArticle

Abstract

In the present work we aimed at comparing the effect of benzothiadiazole (BTH) treatment on defence reactions of sunflower plants to downy mildew and white rot diseases. BTH treatment resulted in reduced disease symptoms in biotrophic and in the early stage of the necrotrophic interactions. To get a better insight into the effect of BTH, changes in the activities of polyphenol oxidase and peroxidase enzymes, as well as the expression of the host response-associated sunflower genes were examined in the plants. Inoculation with Plasmopara halstedii enhanced the polyphenol oxidase and peroxidase enzyme activities, while inoculation with Sclerotinia sclerotiorum did it only at 4 dpi. However, most importantly, in each case extracts from BTH pretreated and inoculated plants showed the highest polyphenol oxidase and peroxidase enzyme activities. Similarly, the accumulation of GST and PDF transcripts was detected following inoculations with both biotrophic and necrotrophic pathogens, and again, BTH pre-treatment enhanced GST and defensine gene activities in the inoculated plants. We suggest that induction of enzyme activities, as well as of the elevated expression of GST, PDF and PR5 genes by BTH pre-treatment may be a significant part of the induced resistance of sunflower to downy mildew and white rot (white mold).

Original languageEnglish
Pages (from-to)13-27
Number of pages15
JournalActa Phytopathologica et Entomologica Hungarica
Volume51
Issue number1
DOIs
Publication statusPublished - Jun 1 2016

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Helianthus annuus
catechol oxidase
peroxidase
downy mildew
enzyme activity
pretreatment
Plasmopara halstedii
genes
Sclerotinia sclerotiorum
induced resistance
molds (fungi)
signs and symptoms (plants)
pathogens
extracts
enzymes

Keywords

  • Downy mildew
  • Glutathione s-transferase
  • Guaiacol peroxidase
  • Plasmopara halstedii
  • Polyphenol oxidase
  • Sclerotinia sclerotiorum
  • White rot

ASJC Scopus subject areas

  • Insect Science
  • Plant Science

Cite this

Induction of resistance with benzothiadiazole in sunflower : A comparison of biotrophic vs. necrotrophic pathosystems. / Körösi, K.; Virányi, F.; Barna, B.; Bán, R.

In: Acta Phytopathologica et Entomologica Hungarica, Vol. 51, No. 1, 01.06.2016, p. 13-27.

Research output: Contribution to journalArticle

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