Induction of human cytomegalovirus (HCMV)-glycoprotein B (gB)-specific neutralizing antibody and phosphoprotein 65 (pp65)-specific cytotoxic T lymphocyte responses by naked DNA immunization

V. Endrész, Laszlo Kari, Klara Berencsi, Csaba Kari, Zsofia Gyulai, Csaba Jeney, Steve Pincus, Ulrich Rodeck, Claude Méric, Stanley A. Plotkin, E. Gönczöl

Research output: Contribution to journalArticle

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Abstract

Plasmids expressing the human cytomegalovirus (HCMV) glycoprotein B (gB) (UL55) or phosphoprotein 65 (pp65) (UL83) were constructed and evaluated for their ability to induce immune responses in mice. The full-length gB as well as a truncated form expressing amino acids 1-680 of gB, and lacking the fragment encoding amino acids 681-907 including the transmembrane domain of gB (gB680) were evaluated. Immunization of mice with plasmids coding for gB or gB680 induced ELISA and neutralizing antibodies, with the highest titres in mice immunized with the gB680 plasmid. Mice immunized with the gB plasmid predominantly produced IgG2a gB-specific antibody, while the gB680 plasmid raised mostly IgG1 anti-gB antibody. Mice immunized with the pp65 plasmid developed pp65-specific cytotoxic T lymphocytes (CTL) and ELISA antibodies. Immunization with a mixture of both gB and pp65 plasmids raised antibodies to both proteins and pp65-specific CTL, indicating a lack of interference between these two plasmids. These results suggest that DNA immunization is a useful approach for vaccination against HCMV disease.

Original languageEnglish
Pages (from-to)50-58
Number of pages9
JournalVaccine
Volume17
Issue number1
DOIs
Publication statusPublished - Jan 1999

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Human herpesvirus 5
phosphoproteins
cytotoxic T-lymphocytes
Phosphoproteins
Cytotoxic T-Lymphocytes
Neutralizing Antibodies
neutralizing antibodies
glycoproteins
Immunization
Glycoproteins
immunization
Plasmids
plasmids
DNA
mice
Antibodies
antibodies
Enzyme-Linked Immunosorbent Assay
Amino Acids
enzyme-linked immunosorbent assay

Keywords

  • Antibody
  • CTL
  • DNA immunization
  • HCMV

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Induction of human cytomegalovirus (HCMV)-glycoprotein B (gB)-specific neutralizing antibody and phosphoprotein 65 (pp65)-specific cytotoxic T lymphocyte responses by naked DNA immunization. / Endrész, V.; Kari, Laszlo; Berencsi, Klara; Kari, Csaba; Gyulai, Zsofia; Jeney, Csaba; Pincus, Steve; Rodeck, Ulrich; Méric, Claude; Plotkin, Stanley A.; Gönczöl, E.

In: Vaccine, Vol. 17, No. 1, 01.1999, p. 50-58.

Research output: Contribution to journalArticle

Endrész, V. ; Kari, Laszlo ; Berencsi, Klara ; Kari, Csaba ; Gyulai, Zsofia ; Jeney, Csaba ; Pincus, Steve ; Rodeck, Ulrich ; Méric, Claude ; Plotkin, Stanley A. ; Gönczöl, E. / Induction of human cytomegalovirus (HCMV)-glycoprotein B (gB)-specific neutralizing antibody and phosphoprotein 65 (pp65)-specific cytotoxic T lymphocyte responses by naked DNA immunization. In: Vaccine. 1999 ; Vol. 17, No. 1. pp. 50-58.
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