Induction of endothelial cell injury by cigarette smoke

J. Nagy, Eugene G. Demaster, I. Wittmann, Pamela Shultz, Leopoldo Raij

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Cigarette smoke contains different populations of free radicals which may be responsible for endothelial cell (EC) injury of smokers. The purpose of this study was to examine the effects of gas-phase cigarette smoke on EC endothelium-derived relaxing factor (EDRF)/NO-guanylate late cyclase (GC)-cGMP pathway and on EC detatchment-type injury after incubation with smoke. Furthermore, we examined whether different kind of antioxidants can prevent smoke-caused EC injury. We measured cGMP pathway using direct (sodium nitroprusside, SNP) and indirect (A23187, the calcium ionophore and bradykinin, BK) activators of GC. Directly and indirectly stimulated EC cGMP production dose-dependently decreased and EC detatchment increased after incubation with smoke. Externally added thiols (glutathione, GSH; D-Penicillamine, DP; N-acetylcysteine, NAC) protected EC from damage of cGMP production and cell detatchment. Other antioxidants (catalase, deferoxamine and superoxide dismutase) were ineffective. These results suggest that the thiol containing GC in EC is destroyed or inactivated or thiol like species responsible for activation of GC is incomplete in EC after incubation with smoke. It is also possible that externally added thiols bind an unknown component of smoke and this way, EC is protected. EC injury may contribute to vascular diseases associated with cigarette smoking.

Original languageEnglish
Pages (from-to)251-263
Number of pages13
JournalEndothelium: Journal of Endothelial Cell Research
Volume5
Issue number4
Publication statusPublished - 1997

Fingerprint

Smoke
Tobacco Products
Endothelial Cells
Wounds and Injuries
Guanylate Cyclase
Sulfhydryl Compounds
Antioxidants
Endothelium-Dependent Relaxing Factors
Penicillamine
Deferoxamine
Calcium Ionophores
Calcimycin
Acetylcysteine
Nitroprusside
Bradykinin
Vascular Diseases
Catalase
Superoxide Dismutase
Free Radicals
Glutathione

Keywords

  • Antioxidants
  • Cigarette smoking
  • EDRF/NO
  • Endothelial cells
  • Guanylate cyclase
  • Thiols

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Induction of endothelial cell injury by cigarette smoke. / Nagy, J.; Demaster, Eugene G.; Wittmann, I.; Shultz, Pamela; Raij, Leopoldo.

In: Endothelium: Journal of Endothelial Cell Research, Vol. 5, No. 4, 1997, p. 251-263.

Research output: Contribution to journalArticle

Nagy, J. ; Demaster, Eugene G. ; Wittmann, I. ; Shultz, Pamela ; Raij, Leopoldo. / Induction of endothelial cell injury by cigarette smoke. In: Endothelium: Journal of Endothelial Cell Research. 1997 ; Vol. 5, No. 4. pp. 251-263.
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