Induction of Cyclooxygenase-2 Following Anoxic Stress in Piglet Cerebral Arteries

David W. Busija, Clara Thore, Tracy Beasley, F. Bari

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Objective: Ischemic stress causes damage to cerebrovascular endothelium and alters arteriolar responses to prostanoid-dependent stimuli. However, effects of ischemic stress on cyclooxygenase (COX) levels in endothelium are unclear. We examined the effect of ischemia and reperfusion and asphyxia and reventilation on production of COX isoforms in cerebral vascular endothelium. Methods: Neonatal pigs were exposed to global ischemia (n = 4) or asphyxia (n = 3) for 5-10 min. Following 2-6 h of recovery, the animals were killed, and the cerebral arteries and arterioles were removed. Cerebral arteries and arterioles were also removed from untreated control animals (n = 1) and from time control animals (n = 3). Cerebral vessels were fixed in 4% formalin and paraffin embedded, and constitutive and inducible COX (COX-1 and COX-2, respectively) levels were assessed using indirect immunofluorescence. Results: Hemotoxylin and eosin staining indicated that anoxic stress leads to enlargement of endothelial cells. Immunofluorescence for COX-1 in endothelium was minimal in cerebral arteries and arterioles from control animals and did not show an increase in animals exposed to anoxic stress. Similarly, cerebral vessels from control animals showed little immunostaining for COX-2. In contrast, immunofluorescence for COX-2 was greatly increased in cerebral arteries and arterioles from animals exposed to asphyxia or ischemia. Conclusions: We conclude that anoxic stress increases COX-2 but not COX-1 levels in cerebral endothelium.

Original languageEnglish
Pages (from-to)379-386
Number of pages8
JournalMicrocirculation
Volume3
Issue number4
Publication statusPublished - Dec 1996

Fingerprint

Cerebral Arteries
Cyclooxygenase 2
Arterioles
Cyclooxygenase 1
Endothelium
Asphyxia
Prostaglandin-Endoperoxide Synthases
Ischemia
Fluorescent Antibody Technique
Vascular Endothelium
Hematoxylin
Eosine Yellowish-(YS)
Indirect Fluorescent Antibody Technique
Paraffin
Formaldehyde
Reperfusion
Prostaglandins
Protein Isoforms
Swine
Endothelial Cells

Keywords

  • Asphyxia
  • Cerebral blood vessels
  • Endothelium
  • Ischemia
  • Prostaglandin H synthase

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Cardiology and Cardiovascular Medicine

Cite this

Induction of Cyclooxygenase-2 Following Anoxic Stress in Piglet Cerebral Arteries. / Busija, David W.; Thore, Clara; Beasley, Tracy; Bari, F.

In: Microcirculation, Vol. 3, No. 4, 12.1996, p. 379-386.

Research output: Contribution to journalArticle

Busija, DW, Thore, C, Beasley, T & Bari, F 1996, 'Induction of Cyclooxygenase-2 Following Anoxic Stress in Piglet Cerebral Arteries', Microcirculation, vol. 3, no. 4, pp. 379-386.
Busija, David W. ; Thore, Clara ; Beasley, Tracy ; Bari, F. / Induction of Cyclooxygenase-2 Following Anoxic Stress in Piglet Cerebral Arteries. In: Microcirculation. 1996 ; Vol. 3, No. 4. pp. 379-386.
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