Induction of cellular immunity to a mycobacterial antigen adsorbed on lamellar particles of lactide polymers

N. Venkataprasad, A. G A Coombes, M. Singh, M. Rohde, K. Wilkinson, F. Hudecz, S. S. Davis, H. M. Vordermeier

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Microspheres prepared from synthetic, biodegradable poly (L-lactide) [PLA] and copolymers of lactide and glycolide such as poly (DL lactide co- glycolide) [PLG] have been widely investigated for controlled delivery of encapsulated vaccine antigens. In this study we describe novel lamellar microparticles produced from PLA to which protein antigens can be adsorbed. These particles when administered to mice, induced strong Th1-type T cell responses to the adsorbed 38 kDa protein antigen from M. tuberculosis characterised by high levels of Interferon-gamma. In addition to proteins, we were also able to adsorb synthetic peptides resulting in specific T cell proliferation. Induction of strong cellular immunity together with the versatility of antigen adsorption to these particles should make such lamellae a useful tool to deliver protective antigens from intracellular pathogens.

Original languageEnglish
Pages (from-to)1814-1819
Number of pages6
JournalVaccine
Volume17
Issue number15-16
DOIs
Publication statusPublished - Apr 9 1999

Fingerprint

Cellular Immunity
cell-mediated immunity
polymers
Polymers
antigens
Antigens
T-Lymphocytes
Polyglactin 910
T-lymphocytes
Th1 Cells
Microspheres
biodegradability
composite polymers
proteins
synthetic peptides
Adsorption
Interferon-gamma
interferon-gamma
Proteins
Vaccines

Keywords

  • Delivery system
  • Lactide polymers
  • Resorbable lamellae
  • Tuberculosis

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Venkataprasad, N., Coombes, A. G. A., Singh, M., Rohde, M., Wilkinson, K., Hudecz, F., ... Vordermeier, H. M. (1999). Induction of cellular immunity to a mycobacterial antigen adsorbed on lamellar particles of lactide polymers. Vaccine, 17(15-16), 1814-1819. https://doi.org/10.1016/S0264-410X(98)00372-7

Induction of cellular immunity to a mycobacterial antigen adsorbed on lamellar particles of lactide polymers. / Venkataprasad, N.; Coombes, A. G A; Singh, M.; Rohde, M.; Wilkinson, K.; Hudecz, F.; Davis, S. S.; Vordermeier, H. M.

In: Vaccine, Vol. 17, No. 15-16, 09.04.1999, p. 1814-1819.

Research output: Contribution to journalArticle

Venkataprasad, N, Coombes, AGA, Singh, M, Rohde, M, Wilkinson, K, Hudecz, F, Davis, SS & Vordermeier, HM 1999, 'Induction of cellular immunity to a mycobacterial antigen adsorbed on lamellar particles of lactide polymers', Vaccine, vol. 17, no. 15-16, pp. 1814-1819. https://doi.org/10.1016/S0264-410X(98)00372-7
Venkataprasad, N. ; Coombes, A. G A ; Singh, M. ; Rohde, M. ; Wilkinson, K. ; Hudecz, F. ; Davis, S. S. ; Vordermeier, H. M. / Induction of cellular immunity to a mycobacterial antigen adsorbed on lamellar particles of lactide polymers. In: Vaccine. 1999 ; Vol. 17, No. 15-16. pp. 1814-1819.
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