Induction of acrosome reaction in dog sperm by calcium ionophore

F. Szász, F. P. Cheng, A. Marks, B. Colenbrander, L. Solti

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The sensitivity of the plasma membrane to calcium ionophore (A23187) challenge was studied in dog sperm using fluorescein lectin staining for the assessment of acrosomal status and viability. Second fraction ejaculates from 5 dogs were washed, resuspended in Ca2+-free (EDTA-treated), 50, 100, 500, 1000 and 2000 μM/l Ca2+-containing Sp-TALP medium and induced with 50, 250, 500, 1000, 2500 and 5000 nM/l calcium ionophore. Samples were collected from each aliquot after 30 and 60 min of induction to assess the percentage of acrosome reacted sperm cells (AR rate), viability and motility by fluorescein isothiocyanate conjugated peanut agglutinin (FITC-PNA) and ethidium-homodimer combined staining. On each slide, 200 sperm cells were assessed under epifluorescence microscope (x 1250) in a blind manner. The response to ionophore challenge (AR rate, viability, motility) varied with Ca2+ and ionophore concentration in the suspension. A significantly higher AR rate was detected in samples containing 100, 500, 1000 and 2000 μM/l Ca2+ (> 40%) than in that containing 50 μM/l. Acrosome reaction could not be successfully induced in the EDTA-treated sample and in any of the aliquots in which 50, 250 and 500 nM/l ionophore concentrations were used for induction. Motility decreased drastically in all of the treated samples and stopped in that sample where a significant AR rate could be detected. Viability remained high (> 75%) during the incubation and did not differ significantly in the treated and the control groups.

Original languageEnglish
Pages (from-to)177-187
Number of pages11
JournalActa Veterinaria Hungarica
Volume45
Issue number2
Publication statusPublished - 1997

Fingerprint

Acrosome Reaction
acrosome reaction
Calcium Ionophores
ionophores
Spermatozoa
Acrosome
spermatozoa
acrosome
Dogs
calcium
dogs
Ionophores
EDTA (chelating agent)
fluorescein
Edetic Acid
Cell Movement
cell viability
Cell Survival
sampling
viability

Keywords

  • Acrosome reaction
  • Calcium ionophore
  • Dog sperm
  • FITC-PNA

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Szász, F., Cheng, F. P., Marks, A., Colenbrander, B., & Solti, L. (1997). Induction of acrosome reaction in dog sperm by calcium ionophore. Acta Veterinaria Hungarica, 45(2), 177-187.

Induction of acrosome reaction in dog sperm by calcium ionophore. / Szász, F.; Cheng, F. P.; Marks, A.; Colenbrander, B.; Solti, L.

In: Acta Veterinaria Hungarica, Vol. 45, No. 2, 1997, p. 177-187.

Research output: Contribution to journalArticle

Szász, F, Cheng, FP, Marks, A, Colenbrander, B & Solti, L 1997, 'Induction of acrosome reaction in dog sperm by calcium ionophore', Acta Veterinaria Hungarica, vol. 45, no. 2, pp. 177-187.
Szász F, Cheng FP, Marks A, Colenbrander B, Solti L. Induction of acrosome reaction in dog sperm by calcium ionophore. Acta Veterinaria Hungarica. 1997;45(2):177-187.
Szász, F. ; Cheng, F. P. ; Marks, A. ; Colenbrander, B. ; Solti, L. / Induction of acrosome reaction in dog sperm by calcium ionophore. In: Acta Veterinaria Hungarica. 1997 ; Vol. 45, No. 2. pp. 177-187.
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