Inducing secondary metabolite production by the endophytic fungus Chaetomium sp. through fungal–bacterial co-culture and epigenetic modification

Sergi Herve Akone, Attila Mándi, T. Kurtán, Rudolf Hartmann, Wenhan Lin, Georgios Daletos, Peter Proksch

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Co-culturing the fungal endophyte Chaetomium sp. with the bacterium Bacillus subtilis on solid rice medium resulted in an up to 8.3-fold increase in the accumulation of constitutively present metabolites that included a 1:1 mixture of 3- and 4-hydroxybenzoic acid methyl esters (1 and 2, respectively), and the polyketides acremonisol A (3), SB236050 (4), and SB238569 (5). In addition, seven compounds including isosulochrin (6), protocatechuic acid methyl ester (7), as well as five new natural products (8–12) were detected in the co-cultures, but not in axenic fungal cultures. Treatment of Chaetomium sp. with the epigenetic modifier suberoylanilide hydroxamic acid or 5-azacytidine resulted in an enhanced accumulation of 6, which was likewise detected during co-culture. Compound 5 showed strong cytotoxicity against the mouse lymphoma L5178Y cell line with an IC50 value of 1 μM, as well as weak antibacterial activity against B. subtilis with an MIC value of 53 μM.

Original languageEnglish
Pages (from-to)6340-6347
Number of pages8
JournalTetrahedron
Volume72
Issue number41
DOIs
Publication statusPublished - Oct 13 2016

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Keywords

  • Bacillus subtilis
  • Chaetomium sp.
  • Co-cultivation
  • Epigenetic modifiers
  • Natural products

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

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