Inducement and reversal of tetracycline resistance in Escherichia coli K-12 and expression of proton gradient-dependent multidrug efflux pump genes

Miguel Viveiros, Ana Jesus, Mafalda Brito, Clara Leandro, Marta Martins, Diane Ordway, Ana Maria Molnar, Joseph Molnar, Leonard Amaral

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Expression of eight transporter genes of Escherichia coli K-12 and its ΔacrAB mutant prior to and after induction of both strains to tetracycline resistance and after reversal of induced resistance were analyzed by quantitative reverse transcriptase PCR. All transporter genes were overexpressed after induced resistance with acrF being 80-fold more expressed in the ΔacrAB tetracycline-induced strain.

Original languageEnglish
Pages (from-to)3578-3582
Number of pages5
JournalAntimicrobial agents and chemotherapy
Volume49
Issue number8
DOIs
Publication statusPublished - Aug 1 2005

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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