Induced expression of the antimicrobial peptide melittin inhibits experimental infection by Mycoplasma gallisepticum in chickens

Vassili N. Lazarev, L. Stipkovits, Judit Biro, Dora Miklodi, Marina M. Shkarupeta, Galina A. Titova, Tatiana A. Akopian, Vadim M. Govorun

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The in vivo action of the antimicrobial peptide melittin, expressed from a recombinant plasmid vector, on chickens experimentally infected with Mycoplasma gallisepticum was studied. The plasmid vector pBI/mel2/rtTA includes the melittin gene under the control of an inducible tetracycline-dependent human cytomegalovirus promoter and the gene coding for the trans-activation protein rtTA. Aerosol administration of the vector, followed by infecting the chickens with M. gallisepticum 1226, is shown to inhibit development of infection. The inhibitory action was confirmed by a complex of clinical, pathomorphological, histological and serological studies, and also by comparing the M. gallisepticum reisolation frequency from the respiratory tract and internal organs. The data suggest that plasmid vectors expressing genes of antimicrobial peptides can be considered as potential agents for the prevention and treatment of mycoplasma infections in poultry farming.

Original languageEnglish
Pages (from-to)536-541
Number of pages6
JournalMicrobes and Infection
Volume6
Issue number6
DOIs
Publication statusPublished - May 2004

Fingerprint

Mycoplasma gallisepticum
Melitten
Chickens
Plasmids
Peptides
Infection
Genes
Mycoplasma Infections
Poultry
Tetracycline
Aerosols
Agriculture
Cytomegalovirus
Respiratory System
Proteins

Keywords

  • Melittin
  • Mycoplasma gallisepticum
  • Recombinant plasmid vector

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Induced expression of the antimicrobial peptide melittin inhibits experimental infection by Mycoplasma gallisepticum in chickens. / Lazarev, Vassili N.; Stipkovits, L.; Biro, Judit; Miklodi, Dora; Shkarupeta, Marina M.; Titova, Galina A.; Akopian, Tatiana A.; Govorun, Vadim M.

In: Microbes and Infection, Vol. 6, No. 6, 05.2004, p. 536-541.

Research output: Contribution to journalArticle

Lazarev, Vassili N. ; Stipkovits, L. ; Biro, Judit ; Miklodi, Dora ; Shkarupeta, Marina M. ; Titova, Galina A. ; Akopian, Tatiana A. ; Govorun, Vadim M. / Induced expression of the antimicrobial peptide melittin inhibits experimental infection by Mycoplasma gallisepticum in chickens. In: Microbes and Infection. 2004 ; Vol. 6, No. 6. pp. 536-541.
@article{7690564401a64d6c88feb65b181e1ecc,
title = "Induced expression of the antimicrobial peptide melittin inhibits experimental infection by Mycoplasma gallisepticum in chickens",
abstract = "The in vivo action of the antimicrobial peptide melittin, expressed from a recombinant plasmid vector, on chickens experimentally infected with Mycoplasma gallisepticum was studied. The plasmid vector pBI/mel2/rtTA includes the melittin gene under the control of an inducible tetracycline-dependent human cytomegalovirus promoter and the gene coding for the trans-activation protein rtTA. Aerosol administration of the vector, followed by infecting the chickens with M. gallisepticum 1226, is shown to inhibit development of infection. The inhibitory action was confirmed by a complex of clinical, pathomorphological, histological and serological studies, and also by comparing the M. gallisepticum reisolation frequency from the respiratory tract and internal organs. The data suggest that plasmid vectors expressing genes of antimicrobial peptides can be considered as potential agents for the prevention and treatment of mycoplasma infections in poultry farming.",
keywords = "Melittin, Mycoplasma gallisepticum, Recombinant plasmid vector",
author = "Lazarev, {Vassili N.} and L. Stipkovits and Judit Biro and Dora Miklodi and Shkarupeta, {Marina M.} and Titova, {Galina A.} and Akopian, {Tatiana A.} and Govorun, {Vadim M.}",
year = "2004",
month = "5",
doi = "10.1016/j.micinf.2004.02.006",
language = "English",
volume = "6",
pages = "536--541",
journal = "Microbes and Infection",
issn = "1286-4579",
publisher = "Elsevier Masson SAS",
number = "6",

}

TY - JOUR

T1 - Induced expression of the antimicrobial peptide melittin inhibits experimental infection by Mycoplasma gallisepticum in chickens

AU - Lazarev, Vassili N.

AU - Stipkovits, L.

AU - Biro, Judit

AU - Miklodi, Dora

AU - Shkarupeta, Marina M.

AU - Titova, Galina A.

AU - Akopian, Tatiana A.

AU - Govorun, Vadim M.

PY - 2004/5

Y1 - 2004/5

N2 - The in vivo action of the antimicrobial peptide melittin, expressed from a recombinant plasmid vector, on chickens experimentally infected with Mycoplasma gallisepticum was studied. The plasmid vector pBI/mel2/rtTA includes the melittin gene under the control of an inducible tetracycline-dependent human cytomegalovirus promoter and the gene coding for the trans-activation protein rtTA. Aerosol administration of the vector, followed by infecting the chickens with M. gallisepticum 1226, is shown to inhibit development of infection. The inhibitory action was confirmed by a complex of clinical, pathomorphological, histological and serological studies, and also by comparing the M. gallisepticum reisolation frequency from the respiratory tract and internal organs. The data suggest that plasmid vectors expressing genes of antimicrobial peptides can be considered as potential agents for the prevention and treatment of mycoplasma infections in poultry farming.

AB - The in vivo action of the antimicrobial peptide melittin, expressed from a recombinant plasmid vector, on chickens experimentally infected with Mycoplasma gallisepticum was studied. The plasmid vector pBI/mel2/rtTA includes the melittin gene under the control of an inducible tetracycline-dependent human cytomegalovirus promoter and the gene coding for the trans-activation protein rtTA. Aerosol administration of the vector, followed by infecting the chickens with M. gallisepticum 1226, is shown to inhibit development of infection. The inhibitory action was confirmed by a complex of clinical, pathomorphological, histological and serological studies, and also by comparing the M. gallisepticum reisolation frequency from the respiratory tract and internal organs. The data suggest that plasmid vectors expressing genes of antimicrobial peptides can be considered as potential agents for the prevention and treatment of mycoplasma infections in poultry farming.

KW - Melittin

KW - Mycoplasma gallisepticum

KW - Recombinant plasmid vector

UR - http://www.scopus.com/inward/record.url?scp=2542422999&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2542422999&partnerID=8YFLogxK

U2 - 10.1016/j.micinf.2004.02.006

DO - 10.1016/j.micinf.2004.02.006

M3 - Article

C2 - 15158186

AN - SCOPUS:2542422999

VL - 6

SP - 536

EP - 541

JO - Microbes and Infection

JF - Microbes and Infection

SN - 1286-4579

IS - 6

ER -