Indomethacin prevents the induction of inducible nitric oxide synthase in murine peritoneal macrophages and decreases their nitric oxide production

A. Hrabák, Vincent Vercruysse, Ilona L. Kahán, Bernard Vray

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Indomethacin (0.1-0.5 mM concentration) inhibits nitric oxide production in murine peritoneal macrophages. This was evidenced by measuring both nitrite production or 14C-L-citrulline formation. The inhibition was caused by the diminution of de novo inducible nitric oxide synthase production as demonstrated by Western blotting experiment. The effect of indomethacin after 4 h treatment was irreversible. NO synthase and arginase activities and the uptake of arginine were not directly affected by the drug. Indomethacin also decreased uridine incorporation in macrophages. The effect of indomethacin on the induction of other enzymes (i.e. arginase) was weaker.

Original languageEnglish
Pages (from-to)1923-1930
Number of pages8
JournalLife Sciences
Volume68
Issue number16
DOIs
Publication statusPublished - Mar 9 2001

Fingerprint

Macrophages
Peritoneal Macrophages
Nitric Oxide Synthase Type II
Indomethacin
Nitric Oxide
Arginase
Citrulline
Enzyme Induction
Uridine
Nitrites
Nitric Oxide Synthase
Arginine
Western Blotting
Enzymes
Pharmaceutical Preparations
Experiments

Keywords

  • Indomethacin
  • Inhibition of induction
  • Macrophages
  • Nitric oxide
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Pharmacology

Cite this

Indomethacin prevents the induction of inducible nitric oxide synthase in murine peritoneal macrophages and decreases their nitric oxide production. / Hrabák, A.; Vercruysse, Vincent; Kahán, Ilona L.; Vray, Bernard.

In: Life Sciences, Vol. 68, No. 16, 09.03.2001, p. 1923-1930.

Research output: Contribution to journalArticle

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