Synthetically and Pharmaceutically interesting racemic amino alcohols with two adjacent chiral centres were analysed by means of indirect high performance liquid chromatography. For resolution of the enantiomers, the recently developed chiral derivatizing agent (1R,2R)-1,3-diacetoxy-1-(4-nitrophenyl)-2-propyl isothiocyanate ((R,R)-DANI) was applied. The diastereomeric thioureas produced after derivatization were separated under reversed-phase conditions. Of the organic modifiers applied in the eluent, methanol proved much more effective than acetonitrile.
|Number of pages||15|
|Journal||Journal of Liquid Chromatography and Related Technologies|
|Publication status||Published - Jan 1 2000|
ASJC Scopus subject areas
- Analytical Chemistry
- Pharmaceutical Science
- Clinical Biochemistry