Indirect high performance liquid chromatographic enantioseparation of racemic amino alcohols with 1,3-diacetoxy-1-(4-nitrophenyl)-2-propyl isothiocyanate as derivatizing agent

M. Peter, F. Fulop

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Synthetically and Pharmaceutically interesting racemic amino alcohols with two adjacent chiral centres were analysed by means of indirect high performance liquid chromatography. For resolution of the enantiomers, the recently developed chiral derivatizing agent (1R,2R)-1,3-diacetoxy-1-(4-nitrophenyl)-2-propyl isothiocyanate ((R,R)-DANI) was applied. The diastereomeric thioureas produced after derivatization were separated under reversed-phase conditions. Of the organic modifiers applied in the eluent, methanol proved much more effective than acetonitrile.

Original languageEnglish
Pages (from-to)2459-2473
Number of pages15
JournalJournal of Liquid Chromatography and Related Technologies
Volume23
Issue number16
DOIs
Publication statusPublished - Jan 1 2000

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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