Increasing the glutathione content in a chilling-sensitive maize genotype using safeners increased protection against chilling-induced injury

G. Kocsy, P. Von Ballmoos, A. Rüegsegger, G. Szalai, G. Galiba, C. Brunold

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

With the aim of analyzing their protective function against chilling-induced injury, the pools of glutathione and its precursors, cysteine (Cys) and γ-glutamyl-Cys, were increased in the chilling-sensitive maize (Zea mays) inbred line Penjalinan using a combination of two herbicide safeners. Compared with the controls, the greatest increase in the pool size of the three thiols was detected in the shoots and roots when both safeners were applied at a concentration of 5 μM. This combination increased the relative protection from chilling from 50% to 75%. It is interesting that this increase in the total glutathione (TG) level was accompanied by a rise in glutathione reductase (GR; EC 1.6.4.2) activity. When the most effective safener combination was applied simultaneously with increasing concentrations of buthionine sulfoximine, a specific inhibitor of glutathione synthesis, the total γ-glutamyl-Cys and TG contents and GR activity were decreased to very low levels and relative protection was lowered from 75% to 44%. During chilling, the ratio of reduced to oxidized thiols first decreased independently of the treatments, but increased again to the initial value in safener-treated seedlings after 7 d at 5°C. Taking all results together resulted in a linear relationship between TG and GR and a biphasic relationship between relative protection and GR or TG, thus demonstrating the relevance of the glutathione levels in protecting maize against chilling-induced injury.

Original languageEnglish
Pages (from-to)1147-1156
Number of pages10
JournalPlant Physiology
Volume127
Issue number3
DOIs
Publication statusPublished - 2001

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Zea mays
Glutathione
glutathione
Genotype
corn
genotype
Wounds and Injuries
Cysteine
cysteine
glutathione-disulfide reductase
thiols
Sulfhydryl Compounds
herbicide safeners
Buthionine Sulfoximine
Glutathione Reductase
Herbicides
cold treatment
Seedlings
inbred lines
synthesis

ASJC Scopus subject areas

  • Plant Science

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Increasing the glutathione content in a chilling-sensitive maize genotype using safeners increased protection against chilling-induced injury. / Kocsy, G.; Von Ballmoos, P.; Rüegsegger, A.; Szalai, G.; Galiba, G.; Brunold, C.

In: Plant Physiology, Vol. 127, No. 3, 2001, p. 1147-1156.

Research output: Contribution to journalArticle

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