Increased thyroid hormone activation accompanies the formation of thyroid hormone-dependent negative feedback in developing chicken hypothalamus

P. Mohácsik, T. Füzesi, M. Doleschall, A. Szilvásy-Szabó, P. Vancamp, Hadadi, V. M. Darras, C. Fekete, B. Gereben

Research output: Contribution to journalArticle

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Abstract

The hypothalamic-pituitary-thyroid axis is governed by hypophysiotropic TRH-synthesizing neurons located in the hypothalamic paraventricular nucleus under control of the negative feedback of thyroid hormones. The mechanisms underlying the ontogeny of this phenomenon are poorly understood. We aimed to determine the onset of thyroid hormone-mediated hypothalamic-negative feedback and studied how local hypothalamic metabolism of thyroid hormones could contribute to this process in developing chicken. In situ hybridization revealed that whereas exogenous T4 did not induce a statistically significant inhibition of TRH expression in the paraventricular nucleus at embryonic day (E)19, T4 treatment was effective at 2 days after hatching (P2). In contrast, TRH expression responded to T3 treatment in both age groups. TSHβ mRNA expression in the pituitary responded to T4 in a similar age-dependent manner. Type 2 deiodinase(D2) was expressed from E13 intanycytesof the mediobasal hypothalamus, and its activity increased between E15 and P2 both in the mediobasal hypothalamus and in tanycyte-lacking hypothalamic regions. Nkx2.1 was coexpressed with D2 in E13 and P2 tanycytes and transcription of the cdio2 gene responded to Nkx2.1 in U87 glioma cells, indicating its potential role in the developmental regulation of D2 activity. The T3-degrading D3 enzyme was also detected in tanycytes, but its level was not markedly changed before and after the period of negative feedback acquisition. These findings suggest that increasing the D2-mediated T3 generation during E18-P2 could provide the sufficient local T3 concentration required for the onset of T3-dependent negative feedback in the developing chicken hypothalamus.

Original languageEnglish
Pages (from-to)1211-1221
Number of pages11
JournalEndocrinology
Volume157
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

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Thyroid Hormones
Hypothalamus
Chickens
Ependymoglial Cells
Paraventricular Hypothalamic Nucleus
Glioma
In Situ Hybridization
Thyroid Gland
Age Groups
Neurons
Messenger RNA
Enzymes
Therapeutics
Genes

ASJC Scopus subject areas

  • Endocrinology

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Increased thyroid hormone activation accompanies the formation of thyroid hormone-dependent negative feedback in developing chicken hypothalamus. / Mohácsik, P.; Füzesi, T.; Doleschall, M.; Szilvásy-Szabó, A.; Vancamp, P.; Hadadi; Darras, V. M.; Fekete, C.; Gereben, B.

In: Endocrinology, Vol. 157, No. 3, 01.03.2016, p. 1211-1221.

Research output: Contribution to journalArticle

Mohácsik, P, Füzesi, T, Doleschall, M, Szilvásy-Szabó, A, Vancamp, P, Hadadi, Darras, VM, Fekete, C & Gereben, B 2016, 'Increased thyroid hormone activation accompanies the formation of thyroid hormone-dependent negative feedback in developing chicken hypothalamus', Endocrinology, vol. 157, no. 3, pp. 1211-1221. https://doi.org/10.1210/en.2015-1496
Mohácsik, P. ; Füzesi, T. ; Doleschall, M. ; Szilvásy-Szabó, A. ; Vancamp, P. ; Hadadi ; Darras, V. M. ; Fekete, C. ; Gereben, B. / Increased thyroid hormone activation accompanies the formation of thyroid hormone-dependent negative feedback in developing chicken hypothalamus. In: Endocrinology. 2016 ; Vol. 157, No. 3. pp. 1211-1221.
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