Increased synthesis of vascular endothelial growth factor in allergic airway inflammation in histidine decarboxylase knockout (HDC-/-) mice

Erna Sziksz, G. Kozma, Zsolt István Komlósi, E. Pállinger, Magdolna Kardos, B. Szebeni, G. Losonczy, A. Falus, András Szabó, T. Tulassay, A. Vannay

Research output: Contribution to journalArticle

Abstract

Histamine and vascular endothelial growth factor (VEGF) have been implicated in the pathogenesis of allergic asthma; they enhance inflammation, vascular permeability, and mucus secretion. Histamine was suggested to alter the level of VEGF via the H2 receptors. Here the authors have applied histidine decarboxylase gene-targeted (HDC-/-) mice, lacking histamine, to investigate the effect of histamine deficiency on VEGF expression in an animal model of asthma. HDC-/- and wild-type (WT) mice were sensitized and challenged with ovalbumin (OVA). VEGF mRNA expression and protein level were determined in the lung. Number of VEGF-positive immune cells of bronchoalveolar lavage (BAL) and their intracellular VEGF content were measured by flow cytometry. VEGF protein level in the lung and in the BAL cells was increased in OVA treated (HDC-/-ova as well as in WTova) animals compared to their controls. However, there was no difference in the VEGF levels between HDC -/- or WT animals, either in the lung or in the BAL cells. In conclusion, increased VEGF production of the lung or BAL immune cells can be induced by allergen provocation independently from the genetic background of the animals. These data suggest that VEGF-mediated allergic processes can persist in the absence of histamine.

Original languageEnglish
Pages (from-to)420-430
Number of pages11
JournalExperimental Lung Research
Volume36
Issue number7
DOIs
Publication statusPublished - Aug 2010

Fingerprint

Histidine Decarboxylase
Knockout Mice
Vascular Endothelial Growth Factor A
Inflammation
Bronchoalveolar Lavage
Histamine
Animals
Ovalbumin
Lung
Asthma
Histamine H2 Receptors
Wild Animals
Flow cytometry
Capillary Permeability
Mucus
Allergens
Ovum
Flow Cytometry
Proteins
Animal Models

Keywords

  • Asthma
  • Histamine
  • Histidine decarboxylase knockout
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

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title = "Increased synthesis of vascular endothelial growth factor in allergic airway inflammation in histidine decarboxylase knockout (HDC-/-) mice",
abstract = "Histamine and vascular endothelial growth factor (VEGF) have been implicated in the pathogenesis of allergic asthma; they enhance inflammation, vascular permeability, and mucus secretion. Histamine was suggested to alter the level of VEGF via the H2 receptors. Here the authors have applied histidine decarboxylase gene-targeted (HDC-/-) mice, lacking histamine, to investigate the effect of histamine deficiency on VEGF expression in an animal model of asthma. HDC-/- and wild-type (WT) mice were sensitized and challenged with ovalbumin (OVA). VEGF mRNA expression and protein level were determined in the lung. Number of VEGF-positive immune cells of bronchoalveolar lavage (BAL) and their intracellular VEGF content were measured by flow cytometry. VEGF protein level in the lung and in the BAL cells was increased in OVA treated (HDC-/-ova as well as in WTova) animals compared to their controls. However, there was no difference in the VEGF levels between HDC -/- or WT animals, either in the lung or in the BAL cells. In conclusion, increased VEGF production of the lung or BAL immune cells can be induced by allergen provocation independently from the genetic background of the animals. These data suggest that VEGF-mediated allergic processes can persist in the absence of histamine.",
keywords = "Asthma, Histamine, Histidine decarboxylase knockout, Vascular endothelial growth factor",
author = "Erna Sziksz and G. Kozma and Koml{\'o}si, {Zsolt Istv{\'a}n} and E. P{\'a}llinger and Magdolna Kardos and B. Szebeni and G. Losonczy and A. Falus and Andr{\'a}s Szab{\'o} and T. Tulassay and A. Vannay",
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T1 - Increased synthesis of vascular endothelial growth factor in allergic airway inflammation in histidine decarboxylase knockout (HDC-/-) mice

AU - Sziksz, Erna

AU - Kozma, G.

AU - Komlósi, Zsolt István

AU - Pállinger, E.

AU - Kardos, Magdolna

AU - Szebeni, B.

AU - Losonczy, G.

AU - Falus, A.

AU - Szabó, András

AU - Tulassay, T.

AU - Vannay, A.

PY - 2010/8

Y1 - 2010/8

N2 - Histamine and vascular endothelial growth factor (VEGF) have been implicated in the pathogenesis of allergic asthma; they enhance inflammation, vascular permeability, and mucus secretion. Histamine was suggested to alter the level of VEGF via the H2 receptors. Here the authors have applied histidine decarboxylase gene-targeted (HDC-/-) mice, lacking histamine, to investigate the effect of histamine deficiency on VEGF expression in an animal model of asthma. HDC-/- and wild-type (WT) mice were sensitized and challenged with ovalbumin (OVA). VEGF mRNA expression and protein level were determined in the lung. Number of VEGF-positive immune cells of bronchoalveolar lavage (BAL) and their intracellular VEGF content were measured by flow cytometry. VEGF protein level in the lung and in the BAL cells was increased in OVA treated (HDC-/-ova as well as in WTova) animals compared to their controls. However, there was no difference in the VEGF levels between HDC -/- or WT animals, either in the lung or in the BAL cells. In conclusion, increased VEGF production of the lung or BAL immune cells can be induced by allergen provocation independently from the genetic background of the animals. These data suggest that VEGF-mediated allergic processes can persist in the absence of histamine.

AB - Histamine and vascular endothelial growth factor (VEGF) have been implicated in the pathogenesis of allergic asthma; they enhance inflammation, vascular permeability, and mucus secretion. Histamine was suggested to alter the level of VEGF via the H2 receptors. Here the authors have applied histidine decarboxylase gene-targeted (HDC-/-) mice, lacking histamine, to investigate the effect of histamine deficiency on VEGF expression in an animal model of asthma. HDC-/- and wild-type (WT) mice were sensitized and challenged with ovalbumin (OVA). VEGF mRNA expression and protein level were determined in the lung. Number of VEGF-positive immune cells of bronchoalveolar lavage (BAL) and their intracellular VEGF content were measured by flow cytometry. VEGF protein level in the lung and in the BAL cells was increased in OVA treated (HDC-/-ova as well as in WTova) animals compared to their controls. However, there was no difference in the VEGF levels between HDC -/- or WT animals, either in the lung or in the BAL cells. In conclusion, increased VEGF production of the lung or BAL immune cells can be induced by allergen provocation independently from the genetic background of the animals. These data suggest that VEGF-mediated allergic processes can persist in the absence of histamine.

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KW - Histamine

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