Increased retention of calcium in the dendrites of long-term potentiated CA1 neurons of the hippocampal slice - A combined electrophysiological and electron histochemical study

U. Kuhnt, A. Mihály, L. Siklós, F. Joó

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Abstract

In view of the importance of calcium in the induction of long-term potentiation (LTP), experiments were carried out to localize calcium at the electron microscopic level in the CA1 region of guinea pig hippocampal slices, following high-frequency stimulation of the Schaffer collaterals. Apart from the ultrastructural localization, a semiquantitative method was used to count the calcium-containing deposits in electron micrographs. Significantly more calcium-containing deposits were seen in the dendrites of the stratum radiatum in slices with LTP than in those without it. A moderate increase of the extradendritic deposits was observed, too. The calcium content of the deposits was determined by means of EGTA incubation and X-ray analysis. The presented results, together with the relevant literature data, underline the importance of calcium-activated processes in postsynaptic structures probably involved in the generation of LTP.

Original languageEnglish
Pages (from-to)185-193
Number of pages9
JournalHistochemistry
Volume90
Issue number3
DOIs
Publication statusPublished - May 1988

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Dendrites
Electrons
Calcium
Neurons
Long-Term Potentiation
Egtazic Acid
Hippocampus
Guinea Pigs
X-Rays

ASJC Scopus subject areas

  • Anatomy

Cite this

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T1 - Increased retention of calcium in the dendrites of long-term potentiated CA1 neurons of the hippocampal slice - A combined electrophysiological and electron histochemical study

AU - Kuhnt, U.

AU - Mihály, A.

AU - Siklós, L.

AU - Joó, F.

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N2 - In view of the importance of calcium in the induction of long-term potentiation (LTP), experiments were carried out to localize calcium at the electron microscopic level in the CA1 region of guinea pig hippocampal slices, following high-frequency stimulation of the Schaffer collaterals. Apart from the ultrastructural localization, a semiquantitative method was used to count the calcium-containing deposits in electron micrographs. Significantly more calcium-containing deposits were seen in the dendrites of the stratum radiatum in slices with LTP than in those without it. A moderate increase of the extradendritic deposits was observed, too. The calcium content of the deposits was determined by means of EGTA incubation and X-ray analysis. The presented results, together with the relevant literature data, underline the importance of calcium-activated processes in postsynaptic structures probably involved in the generation of LTP.

AB - In view of the importance of calcium in the induction of long-term potentiation (LTP), experiments were carried out to localize calcium at the electron microscopic level in the CA1 region of guinea pig hippocampal slices, following high-frequency stimulation of the Schaffer collaterals. Apart from the ultrastructural localization, a semiquantitative method was used to count the calcium-containing deposits in electron micrographs. Significantly more calcium-containing deposits were seen in the dendrites of the stratum radiatum in slices with LTP than in those without it. A moderate increase of the extradendritic deposits was observed, too. The calcium content of the deposits was determined by means of EGTA incubation and X-ray analysis. The presented results, together with the relevant literature data, underline the importance of calcium-activated processes in postsynaptic structures probably involved in the generation of LTP.

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