Increased proportion of hematopoietic stem and progenitor cell population in cord blood of neonates born to mothers with gestational diabetes mellitus

Orsolya Hadarits, András Zóka, G. Barna, Zahra Al-Aissa, Klára Rosta, J. Rigó, Alexandra Kautzky-Willer, A. Somogyi, G. Firneisz

Research output: Contribution to journalArticle

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Abstract

We assessed the hematopoietic stem and progenitor cell (HSPC) population in the cord blood of neonates born to mothers with gestational diabetes mellitus (GDM) in a hypothesis generating pilot study, due to that, neonatal polycythemia may be the consequence of GDM pregnancy. Forty-five pregnant women with GDM (last trimester mean HbA1C = 33.9 mmol/mol) and 42 (nondiabetic) control pregnant women were enrolled after their routine 75 g oral glucose tolerance test (OGTT) between the 24th and 28th gestational week (with expected differences in their mean routine clinical characteristics: plasma glucose at OGTT: 0′ = 5.07 vs. 4.62 mM, 120′ = 8.9 vs. 5.76 mM, age = 35.07 vs. 31.66 years, prepregnancy body mass index = 27.9 vs. 23.9 kg/m2, GDM vs. control, respectively) on a voluntary basis after signing the informed consent. EDTA-treated cord blood samples were analyzed by flow cytometry and the software Kaluza1.2 using CD45 and CD34-specific fluorescent antibodies to identify the HSPC population (CD34+ cells within the CD45dim blast gate). The proportion of CD34+CD45dim HSPCs among the nucleated cells was significantly (P <0.05, statistical power = 60.8%) higher in the cord blood samples of neonates born to mothers with GDM (median 0.38%) compared to neonates born to nondiabetic mothers (median 0.32%) and according to treatment types (P <0.05) median: control 0.32%, GDM-diet only 0.37%, GDM-on insulin 0.45%; control versus GDM on insulin (P <0.05). The increased proportion of circulating CD34+CD45dim cells in the cord blood may possibly be related to altered fetal stem cell mobilization in GDM pregnancy, yet these results should be interpreted only as preliminary due to the small sample sizes.

Original languageEnglish
Pages (from-to)13-17
Number of pages5
JournalStem Cells and Development
Volume25
Issue number1
DOIs
Publication statusPublished - Dec 1 2016

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Gestational Diabetes
Hematopoietic Stem Cells
Fetal Blood
Mothers
Newborn Infant
Population
Glucose Tolerance Test
Pregnant Women
Fetal Stem Cells
Hematopoietic Stem Cell Mobilization
Insulin
Pregnancy
Polycythemia
Third Pregnancy Trimester
Informed Consent
Edetic Acid
Sample Size
Flow Cytometry
Body Mass Index
Software

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology

Cite this

Increased proportion of hematopoietic stem and progenitor cell population in cord blood of neonates born to mothers with gestational diabetes mellitus. / Hadarits, Orsolya; Zóka, András; Barna, G.; Al-Aissa, Zahra; Rosta, Klára; Rigó, J.; Kautzky-Willer, Alexandra; Somogyi, A.; Firneisz, G.

In: Stem Cells and Development, Vol. 25, No. 1, 01.12.2016, p. 13-17.

Research output: Contribution to journalArticle

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AU - Barna, G.

AU - Al-Aissa, Zahra

AU - Rosta, Klára

AU - Rigó, J.

AU - Kautzky-Willer, Alexandra

AU - Somogyi, A.

AU - Firneisz, G.

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