Increased placental expression of cannabinoid receptor 1 in preeclampsia: An observational study

Gergely Fügedi, Miklós Molnár, J. Rigó, Júlia Schönléber, I. Kovalszky, A. Molvarec

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: The endocannabinoid system plays a key role in female reproduction, including implantation, decidualization and placentation. In the present study, we aimed to analyze cannabinoid receptor 1 (CB1), CB2 and fatty acid amid hydrolase (FAAH) expressions and localization in normal and preeclamptic placenta, in order to determine whether placental endocannabinoid expression pattern differs between normal pregnancy and preeclampsia. Methods: Eighteen preeclamptic patients and 18 normotensive, healthy pregnant women with uncomplicated pregnancies were involved in our case-control study. We determined CB1, CB2 and FAAH expressions by Western blotting and immunohistochemistry in placental samples collected directly after Cesarean section. Results: CB1 expression semi-quantified by Western blotting was significantly higher in preeclamptic placenta, and these findings were confirmed by immunohistochemistry. CB1 immunoreactivity was markedly stronger in syncytiotrophoblasts, the mesenchymal core, decidua, villous capillary endothelial and smooth muscle cells, as well as in the amnion in preeclamptic samples compared to normal pregnancies. However, we did not find significant differences between preeclamptic and normal placenta in terms of CB2 and FAAH expressions and immunoreactivity. Conclusions: We observed markedly higher expression of CB1 protein in preeclamptic placental tissue. Increased CB1 expression might cause abnormal decidualization and impair trophoblast invasion, thus being involved in the pathogenesis of preeclampsia. Nevertheless, we did not find significant differences between preeclamptic and normal placental tissue regarding CB2 and FAAH expressions. While the detailed pathogenesis of preeclampsia is still unclear, the endocannabinoid system could play a role in the development of the disease.

Original languageEnglish
Article number395
JournalBMC Pregnancy and Childbirth
Volume14
Issue number1
DOIs
Publication statusPublished - Dec 2 2014

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Cannabinoid Receptors
Hydrolases
Pre-Eclampsia
Endocannabinoids
Observational Studies
Fatty Acids
Cannabinoid Receptor CB2
Placenta
Trophoblasts
Pregnancy
Western Blotting
Immunohistochemistry
Decidua
Placentation
Amnion
Cesarean Section
Smooth Muscle Myocytes
Reproduction
Case-Control Studies
Pregnant Women

Keywords

  • Anandamide
  • Endocannabinoid
  • Placenta
  • Preeclampsia
  • Pregnancy

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Increased placental expression of cannabinoid receptor 1 in preeclampsia : An observational study. / Fügedi, Gergely; Molnár, Miklós; Rigó, J.; Schönléber, Júlia; Kovalszky, I.; Molvarec, A.

In: BMC Pregnancy and Childbirth, Vol. 14, No. 1, 395, 02.12.2014.

Research output: Contribution to journalArticle

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