Increased micronutrient content (Zn, Mn) in the 3Mb(4B) wheat- Aegilops biuncialis substitution and 3Mb.4BS translocation identified by GISH and FISH

András Farkas, István Molnár, Sándor Dulai, Sándor Rapi, Vince Oldal, András Cseh, Klaudia Kruppa, M. Molnár-Láng

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

3Mb Triticum aestivum L. (Mv9kr1)-Aegilops biuncialis Vis. (MvGB642) addition lines were crossed with the Chinese Spring ph1b mutant genotype (CSph1b) to produce 3Mb-wheat chromosome rearrangements. In the F3 generation, 3Mb(4B) substitution lines and 3M b.4BS centric fusions were identified with in situ hybridization using repetitive and genomic DNA probes, and with SSR markers. Grain micronutrient analysis showed that the investigated Ae. biuncialis accession MvGB382 and the parental line MvGB642 are suitable gene sources for improving the grain micronutrient content of wheat, as they have higher K, Zn, Fe, and Mn contents. The results suggested that the Ae. biuncialis chromosome 3M b carries genes determining the grain micronutrient content, as the 3Mb.4BS centric fusion had significantly higher Zn and Mn contents compared with the recipient wheat cultivar. As yield-related traits, such as the number of tillers, the length of main spike, and spikelets per main spike, were similar in the 3Mb.4BS centric fusion and the parental wheat genotype, it can be concluded that this line could be used in pre-breeding programs aimed at enriching elite wheat cultivars with essential micronutrients.

Original languageEnglish
Pages (from-to)61-67
Number of pages7
JournalGenome
Volume57
Issue number2
DOIs
Publication statusPublished - 2014

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Micronutrients
Triticum
Chromosomes
Genotype
DNA Probes
Genes
Breeding
In Situ Hybridization

Keywords

  • Aegilops biuncialis
  • Grain micronutrient content
  • Sequential FISH and GISH
  • Triticum aestivum

ASJC Scopus subject areas

  • Biotechnology
  • Genetics
  • Molecular Biology
  • Medicine(all)

Cite this

Increased micronutrient content (Zn, Mn) in the 3Mb(4B) wheat- Aegilops biuncialis substitution and 3Mb.4BS translocation identified by GISH and FISH. / Farkas, András; Molnár, István; Dulai, Sándor; Rapi, Sándor; Oldal, Vince; Cseh, András; Kruppa, Klaudia; Molnár-Láng, M.

In: Genome, Vol. 57, No. 2, 2014, p. 61-67.

Research output: Contribution to journalArticle

Farkas, András ; Molnár, István ; Dulai, Sándor ; Rapi, Sándor ; Oldal, Vince ; Cseh, András ; Kruppa, Klaudia ; Molnár-Láng, M. / Increased micronutrient content (Zn, Mn) in the 3Mb(4B) wheat- Aegilops biuncialis substitution and 3Mb.4BS translocation identified by GISH and FISH. In: Genome. 2014 ; Vol. 57, No. 2. pp. 61-67.
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