Increased levels of platelet activation markers are positively associated with carotid wall thickness and other atherosclerotic risk factors in obese patients

Éva Csongrádi, B. Nagy, Tibor Fulop, Z. Varga, Z. Karányi, M. Magyar, L. Oláh, M. Papp, Andrea Facskó, J. Kappelmayer, G. Paragh, Miklós Káplár

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Abstract

The role of platelets in the development of atherosclerosis and obesityrelated prothrombotic state is still under investigation. In this cross-sectional cohort study, we measured the levels of different platelet activation markers and evaluated their relationship with carotid intimamedia thickness (IMT) along with other atherosclerotic risk factors in obese patients with or without atherosclerotic co-morbidities. We enrolled 154 obese patients, including 98 with either hypertension, type 2 diabetes mellitus or dyslipidaemia, 56 without these co-morbidities and 62 age- and sex-matched healthy controls. Platelet P-selectin expression and the number of platelet-derived microparticles (PMPs) were measured by flow cytometry; soluble P-selectin levels were analysed by ELISA and Thr715Pro P-selectin polymorphism was determined by PCR-RFLP. Carotid IMT was examined by ultrasonography. The levels of platelet activation parameters were significantly elevated in all obese subjects with increased carotid IMT compared to healthy controls. There was no effect of Thr715Pro genotype on soluble P-selectin levels in obese individuals contrary to normal subjects. Significant and positive association was revealed between carotid IMT and platelet P-selectin (p

Original languageEnglish
Pages (from-to)683-692
Number of pages10
JournalThrombosis and Haemostasis
Volume106
Issue number4
DOIs
Publication statusPublished - Oct 2011

Fingerprint

P-Selectin
Platelet Activation
Blood Platelets
Morbidity
Dyslipidemias
Platelet Count
Restriction Fragment Length Polymorphisms
Type 2 Diabetes Mellitus
Ultrasonography
Atherosclerosis
Flow Cytometry
Cohort Studies
Cross-Sectional Studies
Enzyme-Linked Immunosorbent Assay
Genotype
Hypertension
Polymerase Chain Reaction

Keywords

  • Atherosclerosis
  • Carotid intima-media thickness
  • Microparticles
  • Obesity
  • P-selectin
  • Platelets
  • Thr715Pro polymorphism

ASJC Scopus subject areas

  • Hematology

Cite this

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abstract = "The role of platelets in the development of atherosclerosis and obesityrelated prothrombotic state is still under investigation. In this cross-sectional cohort study, we measured the levels of different platelet activation markers and evaluated their relationship with carotid intimamedia thickness (IMT) along with other atherosclerotic risk factors in obese patients with or without atherosclerotic co-morbidities. We enrolled 154 obese patients, including 98 with either hypertension, type 2 diabetes mellitus or dyslipidaemia, 56 without these co-morbidities and 62 age- and sex-matched healthy controls. Platelet P-selectin expression and the number of platelet-derived microparticles (PMPs) were measured by flow cytometry; soluble P-selectin levels were analysed by ELISA and Thr715Pro P-selectin polymorphism was determined by PCR-RFLP. Carotid IMT was examined by ultrasonography. The levels of platelet activation parameters were significantly elevated in all obese subjects with increased carotid IMT compared to healthy controls. There was no effect of Thr715Pro genotype on soluble P-selectin levels in obese individuals contrary to normal subjects. Significant and positive association was revealed between carotid IMT and platelet P-selectin (p",
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T1 - Increased levels of platelet activation markers are positively associated with carotid wall thickness and other atherosclerotic risk factors in obese patients

AU - Csongrádi, Éva

AU - Nagy, B.

AU - Fulop, Tibor

AU - Varga, Z.

AU - Karányi, Z.

AU - Magyar, M.

AU - Oláh, L.

AU - Papp, M.

AU - Facskó, Andrea

AU - Kappelmayer, J.

AU - Paragh, G.

AU - Káplár, Miklós

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AB - The role of platelets in the development of atherosclerosis and obesityrelated prothrombotic state is still under investigation. In this cross-sectional cohort study, we measured the levels of different platelet activation markers and evaluated their relationship with carotid intimamedia thickness (IMT) along with other atherosclerotic risk factors in obese patients with or without atherosclerotic co-morbidities. We enrolled 154 obese patients, including 98 with either hypertension, type 2 diabetes mellitus or dyslipidaemia, 56 without these co-morbidities and 62 age- and sex-matched healthy controls. Platelet P-selectin expression and the number of platelet-derived microparticles (PMPs) were measured by flow cytometry; soluble P-selectin levels were analysed by ELISA and Thr715Pro P-selectin polymorphism was determined by PCR-RFLP. Carotid IMT was examined by ultrasonography. The levels of platelet activation parameters were significantly elevated in all obese subjects with increased carotid IMT compared to healthy controls. There was no effect of Thr715Pro genotype on soluble P-selectin levels in obese individuals contrary to normal subjects. Significant and positive association was revealed between carotid IMT and platelet P-selectin (p

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