Increased Levels of cathepsin B and L, urokinase-type plasminogen activator and its inhibitor type-1 as an early event in gastric carcinogenesis

F. Farinati, L. Herszènyi, M. Plebani, P. Carraro, M. De Paoli, R. Cardin, G. Roveroni, M. Rugge, D. Nitti, W. F. Grigioni, A. D'Errico, R. Naccarato

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Background: Cysteine proteases [cathepsin B (CATB), cathepsin L (CATL)], the serine protease urokinase-type plasminogen activator (UPA) and its inhibitor type-1 (PAI-1) play an important part in cancer invasion. No data are available on the relationship between these proteases and gastric precancerous changes. Aims: To determine CATB, CATL, UPA, PAI-1 in chronic atrophic gastritis, intestinal metaplasia and gastric epithelial dysplasia, as precancerous changes, and to compare these data with those obtained in gastric cancer. Patients: Endoscopic biopsies were obtained from 12 patients with gastric cancer (cancerous tissue), 33 patients with chronic atrophic gastritis (all with intestinal metaplasia and 12 with dysplasia) and from 47 control subjects, for a total of 92 patients. Methods: Antigen concentrations were measured using ELISA methods. Immunohistochemistry was performed using monoclonal anti-CATB and anti-PAI-1 antibodies. Results: CATB, CATL, UPA and PAI-1 were significantly higher in chronic atrophic gastritis than in controls (CATB: P <0.001; CATL: P <0.005; UPA: P <0.000001; PAI-1: P <0.005). The same was observed for cancer. CATB and UPA were significantly higher in chronic atrophic gastritis, with versus without dysplasia (P <0.05). Dysplastic epithelia showed strong immunoreactivity to PAI-1 and CATB. Conclusions: Our study demonstrates that cathepsins, UPA and PAI-1 may have a role not only in the process of cancer invasion, but also in the progression of precancerous changes into cancer.

Original languageEnglish
Pages (from-to)2581-2587
Number of pages7
JournalCarcinogenesis
Volume17
Issue number12
DOIs
Publication statusPublished - 1996

Fingerprint

Cathepsin L
Cathepsin B
Plasminogen Activator Inhibitor 1
Urokinase-Type Plasminogen Activator
Stomach
Carcinogenesis
Atrophic Gastritis
Cathepsin Z
Metaplasia
Stomach Neoplasms
Neoplasms
Cathepsins
Cysteine Proteases
Serine Proteases
Serine
Peptide Hydrolases
Epithelium
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Biopsy

ASJC Scopus subject areas

  • Cancer Research

Cite this

Increased Levels of cathepsin B and L, urokinase-type plasminogen activator and its inhibitor type-1 as an early event in gastric carcinogenesis. / Farinati, F.; Herszènyi, L.; Plebani, M.; Carraro, P.; De Paoli, M.; Cardin, R.; Roveroni, G.; Rugge, M.; Nitti, D.; Grigioni, W. F.; D'Errico, A.; Naccarato, R.

In: Carcinogenesis, Vol. 17, No. 12, 1996, p. 2581-2587.

Research output: Contribution to journalArticle

Farinati, F, Herszènyi, L, Plebani, M, Carraro, P, De Paoli, M, Cardin, R, Roveroni, G, Rugge, M, Nitti, D, Grigioni, WF, D'Errico, A & Naccarato, R 1996, 'Increased Levels of cathepsin B and L, urokinase-type plasminogen activator and its inhibitor type-1 as an early event in gastric carcinogenesis', Carcinogenesis, vol. 17, no. 12, pp. 2581-2587. https://doi.org/10.1093/carcin/17.12.2581
Farinati, F. ; Herszènyi, L. ; Plebani, M. ; Carraro, P. ; De Paoli, M. ; Cardin, R. ; Roveroni, G. ; Rugge, M. ; Nitti, D. ; Grigioni, W. F. ; D'Errico, A. ; Naccarato, R. / Increased Levels of cathepsin B and L, urokinase-type plasminogen activator and its inhibitor type-1 as an early event in gastric carcinogenesis. In: Carcinogenesis. 1996 ; Vol. 17, No. 12. pp. 2581-2587.
@article{46d5dfb639e54d948c02a5ba43d1bd3b,
title = "Increased Levels of cathepsin B and L, urokinase-type plasminogen activator and its inhibitor type-1 as an early event in gastric carcinogenesis",
abstract = "Background: Cysteine proteases [cathepsin B (CATB), cathepsin L (CATL)], the serine protease urokinase-type plasminogen activator (UPA) and its inhibitor type-1 (PAI-1) play an important part in cancer invasion. No data are available on the relationship between these proteases and gastric precancerous changes. Aims: To determine CATB, CATL, UPA, PAI-1 in chronic atrophic gastritis, intestinal metaplasia and gastric epithelial dysplasia, as precancerous changes, and to compare these data with those obtained in gastric cancer. Patients: Endoscopic biopsies were obtained from 12 patients with gastric cancer (cancerous tissue), 33 patients with chronic atrophic gastritis (all with intestinal metaplasia and 12 with dysplasia) and from 47 control subjects, for a total of 92 patients. Methods: Antigen concentrations were measured using ELISA methods. Immunohistochemistry was performed using monoclonal anti-CATB and anti-PAI-1 antibodies. Results: CATB, CATL, UPA and PAI-1 were significantly higher in chronic atrophic gastritis than in controls (CATB: P <0.001; CATL: P <0.005; UPA: P <0.000001; PAI-1: P <0.005). The same was observed for cancer. CATB and UPA were significantly higher in chronic atrophic gastritis, with versus without dysplasia (P <0.05). Dysplastic epithelia showed strong immunoreactivity to PAI-1 and CATB. Conclusions: Our study demonstrates that cathepsins, UPA and PAI-1 may have a role not only in the process of cancer invasion, but also in the progression of precancerous changes into cancer.",
author = "F. Farinati and L. Hersz{\`e}nyi and M. Plebani and P. Carraro and {De Paoli}, M. and R. Cardin and G. Roveroni and M. Rugge and D. Nitti and Grigioni, {W. F.} and A. D'Errico and R. Naccarato",
year = "1996",
doi = "10.1093/carcin/17.12.2581",
language = "English",
volume = "17",
pages = "2581--2587",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "12",

}

TY - JOUR

T1 - Increased Levels of cathepsin B and L, urokinase-type plasminogen activator and its inhibitor type-1 as an early event in gastric carcinogenesis

AU - Farinati, F.

AU - Herszènyi, L.

AU - Plebani, M.

AU - Carraro, P.

AU - De Paoli, M.

AU - Cardin, R.

AU - Roveroni, G.

AU - Rugge, M.

AU - Nitti, D.

AU - Grigioni, W. F.

AU - D'Errico, A.

AU - Naccarato, R.

PY - 1996

Y1 - 1996

N2 - Background: Cysteine proteases [cathepsin B (CATB), cathepsin L (CATL)], the serine protease urokinase-type plasminogen activator (UPA) and its inhibitor type-1 (PAI-1) play an important part in cancer invasion. No data are available on the relationship between these proteases and gastric precancerous changes. Aims: To determine CATB, CATL, UPA, PAI-1 in chronic atrophic gastritis, intestinal metaplasia and gastric epithelial dysplasia, as precancerous changes, and to compare these data with those obtained in gastric cancer. Patients: Endoscopic biopsies were obtained from 12 patients with gastric cancer (cancerous tissue), 33 patients with chronic atrophic gastritis (all with intestinal metaplasia and 12 with dysplasia) and from 47 control subjects, for a total of 92 patients. Methods: Antigen concentrations were measured using ELISA methods. Immunohistochemistry was performed using monoclonal anti-CATB and anti-PAI-1 antibodies. Results: CATB, CATL, UPA and PAI-1 were significantly higher in chronic atrophic gastritis than in controls (CATB: P <0.001; CATL: P <0.005; UPA: P <0.000001; PAI-1: P <0.005). The same was observed for cancer. CATB and UPA were significantly higher in chronic atrophic gastritis, with versus without dysplasia (P <0.05). Dysplastic epithelia showed strong immunoreactivity to PAI-1 and CATB. Conclusions: Our study demonstrates that cathepsins, UPA and PAI-1 may have a role not only in the process of cancer invasion, but also in the progression of precancerous changes into cancer.

AB - Background: Cysteine proteases [cathepsin B (CATB), cathepsin L (CATL)], the serine protease urokinase-type plasminogen activator (UPA) and its inhibitor type-1 (PAI-1) play an important part in cancer invasion. No data are available on the relationship between these proteases and gastric precancerous changes. Aims: To determine CATB, CATL, UPA, PAI-1 in chronic atrophic gastritis, intestinal metaplasia and gastric epithelial dysplasia, as precancerous changes, and to compare these data with those obtained in gastric cancer. Patients: Endoscopic biopsies were obtained from 12 patients with gastric cancer (cancerous tissue), 33 patients with chronic atrophic gastritis (all with intestinal metaplasia and 12 with dysplasia) and from 47 control subjects, for a total of 92 patients. Methods: Antigen concentrations were measured using ELISA methods. Immunohistochemistry was performed using monoclonal anti-CATB and anti-PAI-1 antibodies. Results: CATB, CATL, UPA and PAI-1 were significantly higher in chronic atrophic gastritis than in controls (CATB: P <0.001; CATL: P <0.005; UPA: P <0.000001; PAI-1: P <0.005). The same was observed for cancer. CATB and UPA were significantly higher in chronic atrophic gastritis, with versus without dysplasia (P <0.05). Dysplastic epithelia showed strong immunoreactivity to PAI-1 and CATB. Conclusions: Our study demonstrates that cathepsins, UPA and PAI-1 may have a role not only in the process of cancer invasion, but also in the progression of precancerous changes into cancer.

UR - http://www.scopus.com/inward/record.url?scp=12644288323&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12644288323&partnerID=8YFLogxK

U2 - 10.1093/carcin/17.12.2581

DO - 10.1093/carcin/17.12.2581

M3 - Article

C2 - 9006092

AN - SCOPUS:12644288323

VL - 17

SP - 2581

EP - 2587

JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 12

ER -