Increased frequency of intracellular interleukin (IL)-13 and IL-10, but not IL-4, expressing CD4+ and CD8+ peripheral T cells of patients with atopic dermatitis

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Abstract

Background: A number of studies exist demonstrating the increased expression of type 2 cytokines and decreased capacity to produce interferon-γ (IFN-γ) in peripheral blood mononuclear cells (PBMCs) of patients with atopic dermatitis (AD). Objectives: To clarify the results of recent studies concerning the role of interleukin (IL)-4 and IL-13 in PBMCs of AD patients, we analysed the activation status of lymphocyte subpopulations. Methods: We measured the intracellular expression and serum levels of certain type 1 and type 2 cytokines, using cell surface and intracellular cytokine staining, flow cytometry and enzyme-linked immunosorbent assay techniques. Results: The frequency of IL-10 and IL-13 producing CD4+ and CDS+ T cells was significantly higher in patients with AD, while the frequency of IFN-γ secreting helper and cytotoxic T cells was significantly lower in patients with AD than in control subjects. The serum levels of IL-10 and IL-13 were also significantly increased. There were no significant differences observed between the experimental groups in the frequency of IL-4 producing CD4+ and CD8+ cells. Conclusions: This study demonstrates a type 2 cytokine production in the CD4+ and CD8+ T cells of AD patients, which is characterized by an elevated IL-13, but not by IL-4 secretion, and by an increased level of the immunoregulatory IL-10, which can contribute to a decrease in IFN-γ expression.

Original languageEnglish
Pages (from-to)1135-1141
Number of pages7
JournalBritish Journal of Dermatology
Volume147
Issue number6
DOIs
Publication statusPublished - Dec 1 2002

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Interleukin-13
Atopic Dermatitis
Interleukin-4
Interleukin-10
T-Lymphocytes
Interferons
Cytokines
Blood Cells
Immunosorbent Techniques
Lymphocyte Subsets
Helper-Inducer T-Lymphocytes
Serum
Flow Cytometry
Enzyme-Linked Immunosorbent Assay
Staining and Labeling

Keywords

  • Atopic dermatitis
  • CD4+ cells
  • CD8+ cells
  • Flow cytometry
  • Interleukin-13
  • Interleukin-4

ASJC Scopus subject areas

  • Dermatology

Cite this

@article{6db606e8723f4ac89baebbe2a5ec3642,
title = "Increased frequency of intracellular interleukin (IL)-13 and IL-10, but not IL-4, expressing CD4+ and CD8+ peripheral T cells of patients with atopic dermatitis",
abstract = "Background: A number of studies exist demonstrating the increased expression of type 2 cytokines and decreased capacity to produce interferon-γ (IFN-γ) in peripheral blood mononuclear cells (PBMCs) of patients with atopic dermatitis (AD). Objectives: To clarify the results of recent studies concerning the role of interleukin (IL)-4 and IL-13 in PBMCs of AD patients, we analysed the activation status of lymphocyte subpopulations. Methods: We measured the intracellular expression and serum levels of certain type 1 and type 2 cytokines, using cell surface and intracellular cytokine staining, flow cytometry and enzyme-linked immunosorbent assay techniques. Results: The frequency of IL-10 and IL-13 producing CD4+ and CDS+ T cells was significantly higher in patients with AD, while the frequency of IFN-γ secreting helper and cytotoxic T cells was significantly lower in patients with AD than in control subjects. The serum levels of IL-10 and IL-13 were also significantly increased. There were no significant differences observed between the experimental groups in the frequency of IL-4 producing CD4+ and CD8+ cells. Conclusions: This study demonstrates a type 2 cytokine production in the CD4+ and CD8+ T cells of AD patients, which is characterized by an elevated IL-13, but not by IL-4 secretion, and by an increased level of the immunoregulatory IL-10, which can contribute to a decrease in IFN-γ expression.",
keywords = "Atopic dermatitis, CD4+ cells, CD8+ cells, Flow cytometry, Interleukin-13, Interleukin-4",
author = "M. Aleksza and B. Irinyi and A. Luk{\'a}cs and P. Antal-Szalm{\'a}s and J. Hunyadi and A. Szegedi",
year = "2002",
month = "12",
day = "1",
doi = "10.1046/j.1365-2133.2002.05013.x",
language = "English",
volume = "147",
pages = "1135--1141",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "Wiley-Blackwell",
number = "6",

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TY - JOUR

T1 - Increased frequency of intracellular interleukin (IL)-13 and IL-10, but not IL-4, expressing CD4+ and CD8+ peripheral T cells of patients with atopic dermatitis

AU - Aleksza, M.

AU - Irinyi, B.

AU - Lukács, A.

AU - Antal-Szalmás, P.

AU - Hunyadi, J.

AU - Szegedi, A.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Background: A number of studies exist demonstrating the increased expression of type 2 cytokines and decreased capacity to produce interferon-γ (IFN-γ) in peripheral blood mononuclear cells (PBMCs) of patients with atopic dermatitis (AD). Objectives: To clarify the results of recent studies concerning the role of interleukin (IL)-4 and IL-13 in PBMCs of AD patients, we analysed the activation status of lymphocyte subpopulations. Methods: We measured the intracellular expression and serum levels of certain type 1 and type 2 cytokines, using cell surface and intracellular cytokine staining, flow cytometry and enzyme-linked immunosorbent assay techniques. Results: The frequency of IL-10 and IL-13 producing CD4+ and CDS+ T cells was significantly higher in patients with AD, while the frequency of IFN-γ secreting helper and cytotoxic T cells was significantly lower in patients with AD than in control subjects. The serum levels of IL-10 and IL-13 were also significantly increased. There were no significant differences observed between the experimental groups in the frequency of IL-4 producing CD4+ and CD8+ cells. Conclusions: This study demonstrates a type 2 cytokine production in the CD4+ and CD8+ T cells of AD patients, which is characterized by an elevated IL-13, but not by IL-4 secretion, and by an increased level of the immunoregulatory IL-10, which can contribute to a decrease in IFN-γ expression.

AB - Background: A number of studies exist demonstrating the increased expression of type 2 cytokines and decreased capacity to produce interferon-γ (IFN-γ) in peripheral blood mononuclear cells (PBMCs) of patients with atopic dermatitis (AD). Objectives: To clarify the results of recent studies concerning the role of interleukin (IL)-4 and IL-13 in PBMCs of AD patients, we analysed the activation status of lymphocyte subpopulations. Methods: We measured the intracellular expression and serum levels of certain type 1 and type 2 cytokines, using cell surface and intracellular cytokine staining, flow cytometry and enzyme-linked immunosorbent assay techniques. Results: The frequency of IL-10 and IL-13 producing CD4+ and CDS+ T cells was significantly higher in patients with AD, while the frequency of IFN-γ secreting helper and cytotoxic T cells was significantly lower in patients with AD than in control subjects. The serum levels of IL-10 and IL-13 were also significantly increased. There were no significant differences observed between the experimental groups in the frequency of IL-4 producing CD4+ and CD8+ cells. Conclusions: This study demonstrates a type 2 cytokine production in the CD4+ and CD8+ T cells of AD patients, which is characterized by an elevated IL-13, but not by IL-4 secretion, and by an increased level of the immunoregulatory IL-10, which can contribute to a decrease in IFN-γ expression.

KW - Atopic dermatitis

KW - CD4+ cells

KW - CD8+ cells

KW - Flow cytometry

KW - Interleukin-13

KW - Interleukin-4

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U2 - 10.1046/j.1365-2133.2002.05013.x

DO - 10.1046/j.1365-2133.2002.05013.x

M3 - Article

VL - 147

SP - 1135

EP - 1141

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 6

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