Increased formation of methylglyoxal and protein glycation, oxidation and nitrosation in triosephosphate isomerase deficiency

Naila Ahmed, Sinan Battah, Nikolaos Karachalias, Roya Babaei-Jadidi, M. Horányi, Klára Baróti, S. Hollán, Paul J. Thornalley

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Triosephosphate isomerase deficiency is associated with the accumulation of dihydroxyacetonephosphate (DHAP) to abnormally high levels, congenital haemolytic anaemia and a clinical syndrome of progressive neuromuscular degeneration leading to infant mortality. DHAP degrades spontaneously to methylglyoxal (MG) - a potent precursor of advanced glycation endproducts (AGEs). MG is detoxified to D-lactate intracellularly by the glyoxalase system. We investigated the changes in MG metabolism and markers of protein glycation, oxidation and nitrosation in a Hungarian family with two germline identical brothers, compound heterozygotes for triosephosphate isomerase deficiency, one with clinical manifestations of chronic neurodegeneration and the other neurologically intact. The concentration of MG and activity of glyoxalase I in red blood cells (RBCs) were increased, and the concentrations of D-lactate in blood plasma and D-lactate urinary excretion were also increased markedly in the propositus. There were concomitant increases in MG-derived AGEs and the oxidative marker dityrosine in hemoglobin. Smaller and nonsignificant increases were found in the neurologically unaffected brother and parents. There was a marked increase (15-fold) in urinary excretion of the nitrosative stress marker 3-nitrotyrosine in the propositus. The increased derangement of MG metabolism and associated glycation, oxidative and nitrosative stress in the propositus may be linked to neurodegenerative process in triosephosphate isomerase deficiency.

Original languageEnglish
Pages (from-to)121-132
Number of pages12
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1639
Issue number2
DOIs
Publication statusPublished - Oct 15 2003

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Nitrosation
Pyruvaldehyde
Lactic Acid
Proteins
Congenital Hemolytic Anemia
Lactoylglutathione Lyase
Infant Mortality
Heterozygote
Triosephosphate Isomerase Deficiency
Hemoglobins
Oxidative Stress
Erythrocytes

Keywords

  • 6-aminoquinolyl-N-hydroxysuccinimidyl- carbamate
  • Advanced glycation endproduct
  • AGE
  • AQC
  • CEL
  • Dihydroxyacetonephosphate
  • Glyoxalase
  • Methylglyoxal
  • Neuromuscular degeneration
  • Oxidative stress
  • Triosephosphate isomerase

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Biophysics

Cite this

Increased formation of methylglyoxal and protein glycation, oxidation and nitrosation in triosephosphate isomerase deficiency. / Ahmed, Naila; Battah, Sinan; Karachalias, Nikolaos; Babaei-Jadidi, Roya; Horányi, M.; Baróti, Klára; Hollán, S.; Thornalley, Paul J.

In: Biochimica et Biophysica Acta - Molecular Basis of Disease, Vol. 1639, No. 2, 15.10.2003, p. 121-132.

Research output: Contribution to journalArticle

Ahmed, Naila ; Battah, Sinan ; Karachalias, Nikolaos ; Babaei-Jadidi, Roya ; Horányi, M. ; Baróti, Klára ; Hollán, S. ; Thornalley, Paul J. / Increased formation of methylglyoxal and protein glycation, oxidation and nitrosation in triosephosphate isomerase deficiency. In: Biochimica et Biophysica Acta - Molecular Basis of Disease. 2003 ; Vol. 1639, No. 2. pp. 121-132.
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AU - Ahmed, Naila

AU - Battah, Sinan

AU - Karachalias, Nikolaos

AU - Babaei-Jadidi, Roya

AU - Horányi, M.

AU - Baróti, Klára

AU - Hollán, S.

AU - Thornalley, Paul J.

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AB - Triosephosphate isomerase deficiency is associated with the accumulation of dihydroxyacetonephosphate (DHAP) to abnormally high levels, congenital haemolytic anaemia and a clinical syndrome of progressive neuromuscular degeneration leading to infant mortality. DHAP degrades spontaneously to methylglyoxal (MG) - a potent precursor of advanced glycation endproducts (AGEs). MG is detoxified to D-lactate intracellularly by the glyoxalase system. We investigated the changes in MG metabolism and markers of protein glycation, oxidation and nitrosation in a Hungarian family with two germline identical brothers, compound heterozygotes for triosephosphate isomerase deficiency, one with clinical manifestations of chronic neurodegeneration and the other neurologically intact. The concentration of MG and activity of glyoxalase I in red blood cells (RBCs) were increased, and the concentrations of D-lactate in blood plasma and D-lactate urinary excretion were also increased markedly in the propositus. There were concomitant increases in MG-derived AGEs and the oxidative marker dityrosine in hemoglobin. Smaller and nonsignificant increases were found in the neurologically unaffected brother and parents. There was a marked increase (15-fold) in urinary excretion of the nitrosative stress marker 3-nitrotyrosine in the propositus. The increased derangement of MG metabolism and associated glycation, oxidative and nitrosative stress in the propositus may be linked to neurodegenerative process in triosephosphate isomerase deficiency.

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