Increased expression of hypoxia-inducible factor 1α in coeliac disease

Ádám Vannay, Erna Sziksz, Á PRókai, Gábor Veres, Kriszta Molnár, Dorottya Nagy SZAKál, Anna ÓNóldy, Ilma R. Korponay-Szabó, András Szabó, Tivadar Tulassay, András ARATó, Beáta Szebeni

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Abstract

Previously, it has been suggested that hypoxia-inducible factor (HIF) 1 signaling may play determinative role in the maintenance of the barrier function of the intestinal epithelium in inflammatory bowel disease. Our aim was to depict the alteration of HIF-1α and related genes in celiac disease (CD) where the importance of the barrier function is well known. Duodenal biopsy specimens were collected from 16 children with untreated CD, 9 children with treated CD and 10 controls. HIF-1α, trefoil factor 1 (TFF1), ecto-5-prime nucleotidase (CD73), and multi drug resistance gene 1 (MDR1) mRNA and HIF-1α protein expression were determined by real-time PCR and Western blot, respectively. Localization of HIF-1α was determined by immunofluorescent staining. We found increased HIF-1α and TFF1 mRNA and HIF-1α protein expression in the duodenal mucosa of children with untreated CD compared with controls or children with treated CD (p < 0.05). In untreated CD children, HIF-1α staining was present in cytoplasmic and nuclear region of the villous enterocytes. In treated CD mRNA expression of CD73 and MDR1 were increased compared with controls (p < 0.01 and 0.05, respectively). Our results of increased mucosal HIF-1α expression in CD children suggest the contribution of this signaling pathway in the pathomechanism of CD.

Original languageEnglish
Pages (from-to)118-122
Number of pages5
JournalPediatric research
Volume68
Issue number2
DOIs
Publication statusPublished - Aug 2010

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ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Vannay, Á., Sziksz, E., PRókai, Á., Veres, G., Molnár, K., SZAKál, D. N., ÓNóldy, A., Korponay-Szabó, I. R., Szabó, A., Tulassay, T., ARATó, A., & Szebeni, B. (2010). Increased expression of hypoxia-inducible factor 1α in coeliac disease. Pediatric research, 68(2), 118-122. https://doi.org/10.1203/PDR.0b013e3181e5bc96