Increased expression of epidermal IL-8 receptor in psoriasis

Down-regulation by FK-506 in vitro

Barbara S. Schulz, Günter Michel, Stefan Wagner, Rosita Süss, Axel Beetz, Ralf U. Peter, L. Kemény, Thomas Ruzicka

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

IL-8 is a chemotactic cytokine with proinflammatory and growth-promoting activities. Recently it has been shown to influence several functions of keratinocytes, including HLA-DR expression, chemotaxis, and proliferation by binding to a specific receptor. Because psoriasis vulgaris is characterized by epidermal hyperproliferation and infiltration of inflammatory cells, we investigated the expression of IL-8 and its receptor in normal and psoriatic epidermis using semiquantitative reverse-transcriptase-polymerase chain reaction. In addition the mRNA levels of the proto-oncogenes c-ras, c-raf, c-myc, and HER-2 were also investigated as potential growth-promoting stimuli in psoriatic epidermis. IL-8 mRNA was only detected in lesional psoriatic epidermis, and IL-8R-specific mRNA was found to be 10 times increased in lesional psoriatic epidermis. There was no significant difference in the proto-oncogene mRNA levels. In order to test the relevance of the massively increased IL-8R levels in psoriatic epidermis, we investigated the effect of the antipsoriatic drug FK-506 on specific IL-8 and IL-8R mRNA expression. FK-506 dose dependently inhibited IL-8R expression and function. Our data suggest that in psoriatic skin, elevated IL-8 levels and markedly increased IL-8R expression may act in concert to induce the cardinal signs of psoriasis - epidermal hyperproliferation and leukocyte infiltration. IL-8R may prove a molecular target for antipsoriatic drugs such as FK-506.

Original languageEnglish
Pages (from-to)4399-4406
Number of pages8
JournalJournal of Immunology
Volume151
Issue number8
Publication statusPublished - 1993

Fingerprint

Interleukin-8 Receptors
Tacrolimus
Psoriasis
Epidermis
Interleukin-8
Down-Regulation
Messenger RNA
Proto-Oncogenes
HLA-DR Antigens
Chemotaxis
Reverse Transcriptase Polymerase Chain Reaction
Keratinocytes
Chemokines
Pharmaceutical Preparations
Leukocytes
In Vitro Techniques
Skin
Growth

ASJC Scopus subject areas

  • Immunology

Cite this

Schulz, B. S., Michel, G., Wagner, S., Süss, R., Beetz, A., Peter, R. U., ... Ruzicka, T. (1993). Increased expression of epidermal IL-8 receptor in psoriasis: Down-regulation by FK-506 in vitro. Journal of Immunology, 151(8), 4399-4406.

Increased expression of epidermal IL-8 receptor in psoriasis : Down-regulation by FK-506 in vitro. / Schulz, Barbara S.; Michel, Günter; Wagner, Stefan; Süss, Rosita; Beetz, Axel; Peter, Ralf U.; Kemény, L.; Ruzicka, Thomas.

In: Journal of Immunology, Vol. 151, No. 8, 1993, p. 4399-4406.

Research output: Contribution to journalArticle

Schulz, BS, Michel, G, Wagner, S, Süss, R, Beetz, A, Peter, RU, Kemény, L & Ruzicka, T 1993, 'Increased expression of epidermal IL-8 receptor in psoriasis: Down-regulation by FK-506 in vitro', Journal of Immunology, vol. 151, no. 8, pp. 4399-4406.
Schulz BS, Michel G, Wagner S, Süss R, Beetz A, Peter RU et al. Increased expression of epidermal IL-8 receptor in psoriasis: Down-regulation by FK-506 in vitro. Journal of Immunology. 1993;151(8):4399-4406.
Schulz, Barbara S. ; Michel, Günter ; Wagner, Stefan ; Süss, Rosita ; Beetz, Axel ; Peter, Ralf U. ; Kemény, L. ; Ruzicka, Thomas. / Increased expression of epidermal IL-8 receptor in psoriasis : Down-regulation by FK-506 in vitro. In: Journal of Immunology. 1993 ; Vol. 151, No. 8. pp. 4399-4406.
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