Increase of green plant regeneration efficiency by callus selection in Puccinellia limosa (Schur.) Holmbg.

L. E. Heszky, D. Q. Binh, E. Kiss, G. Gyulai

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Three main types of callus have been selected from seeds of salt marsh grass (Puccinellia limosa (Schur.) Holmbg. ) subcultured on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid and kinetin. Callus type I differentiated only occasionally. Callus type II produced roots but no shoots under all tested culture conditions. Both green (47 %) and albino plants have been obtained from the embryogenic callus type III. Callus type III was divided into two subtypes (greening and non-greening) according to the presence or absence of green spots. Separated greening embryogenic callus gave up to 87 % green plants, whereas non-greening callus produced only 4 %.

Original languageEnglish
Pages (from-to)174-177
Number of pages4
JournalPlant Cell Reports
Volume8
Issue number3
DOIs
Publication statusPublished - Mar 1 1989

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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