Increase in functional activity rather than in amount of Gi-α in failing human heart with dilated cardiomyopathy

Liang Xiong Fu, Qi Ming Liang, Finn Waagstein, Johan Hoebeke, Christer Sylvén, E. Jansson, P. Sótónyi, Åke Hjalmarson

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Objective: The aim was to investigate whether or not increased pertussis toxin catalysed ADP ribosylation correlates with increased amount of Gi-α in failing human heart. Design: Antisera raised against unique synthetic peptides corresponding to α subunits of Gs and Gi 1-3 were used in immunoblotting and elisa to determine amounts of various G proteins. Adenylyl cyclase activity, β adrenoceptors, and muscarinic receptors were then measured in cardiomyopathic hearts (n=6) obtained at transplant in order to study whether or not an altered expression of G proteins has relevance to the integrity and function of the receptor - adenylyl cyclase system. Six non-failing control hearts were also studied. Results: No significant differences in the peptide equivalent amounts of either Gs or Gi were found in the failing human heart as compared to the non-failing heart. However, functional activity of Gi was shown to increase significantly since there was a decrease in basal (57%), isoprenaline stimulated (60%), and guanyliminodiphosphate stimulated (52%) adenylyl cyclase activity. In contrast the density of β adrenoceptors was markedly decreased (51%) in failing human heart in comparison to non-failing hearts. Neither the density nor the affinity of muscarinic receptors changed in the failing human heart. Conclusion: These results suggest that in the failing human heart, there is an increase in functional activity rather than in amount of Gi, and an important part of functional expression of Gi-a may be regulated at the post-translational level.Cardiovascular Research 1992;26:950-955.

Original languageEnglish
Pages (from-to)950-955
Number of pages6
JournalCardiovascular Research
Volume26
Issue number10
DOIs
Publication statusPublished - Jan 1 1992

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Dilated Cardiomyopathy
Adenylyl Cyclases
Muscarinic Receptors
GTP-Binding Proteins
Adrenergic Receptors
Peptides
Pertussis Toxin
Isoproterenol
Immunoblotting
Adenosine Diphosphate
Immune Sera
Transplants

Keywords

  • Adenylyl cyclase
  • Antibody
  • Cardiomyopathy
  • G protein
  • Receptors

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Fu, L. X., Liang, Q. M., Waagstein, F., Hoebeke, J., Sylvén, C., Jansson, E., ... Hjalmarson, Å. (1992). Increase in functional activity rather than in amount of Gi-α in failing human heart with dilated cardiomyopathy. Cardiovascular Research, 26(10), 950-955. https://doi.org/10.1093/cvr/26.10.950

Increase in functional activity rather than in amount of Gi-α in failing human heart with dilated cardiomyopathy. / Fu, Liang Xiong; Liang, Qi Ming; Waagstein, Finn; Hoebeke, Johan; Sylvén, Christer; Jansson, E.; Sótónyi, P.; Hjalmarson, Åke.

In: Cardiovascular Research, Vol. 26, No. 10, 01.01.1992, p. 950-955.

Research output: Contribution to journalArticle

Fu, LX, Liang, QM, Waagstein, F, Hoebeke, J, Sylvén, C, Jansson, E, Sótónyi, P & Hjalmarson, Å 1992, 'Increase in functional activity rather than in amount of Gi-α in failing human heart with dilated cardiomyopathy', Cardiovascular Research, vol. 26, no. 10, pp. 950-955. https://doi.org/10.1093/cvr/26.10.950
Fu, Liang Xiong ; Liang, Qi Ming ; Waagstein, Finn ; Hoebeke, Johan ; Sylvén, Christer ; Jansson, E. ; Sótónyi, P. ; Hjalmarson, Åke. / Increase in functional activity rather than in amount of Gi-α in failing human heart with dilated cardiomyopathy. In: Cardiovascular Research. 1992 ; Vol. 26, No. 10. pp. 950-955.
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AU - Sylvén, Christer

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AU - Sótónyi, P.

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