Increase in Alzheimer's related markers preceeds memory disturbances: Studies in vasopressin-deficient Brattleboro rat

János Varga, Barbara Klausz, Ágnes Domokos, Sára Kálmán, M. Pákáski, Szabina Szucs, Dénes Garab, A. Zvara, L. Puskás, J. Kálmán, Júlia Tímár, G. Bagdy, D. Zelena

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Alzheimer's disease (AD) is the most common form of dementia in the elderly. For more effective therapy early diagnostic markers could be beneficial. Therefore we compared one year old rats with adults and examined if changes in possible brain markers of AD preceeded memory decline. We also tested if vasopressin-deficient animals were useful model of AD as vasopressin has well known positive effect on memory and AD patient has decreased vasopressin production.We compared adult (3 month) and old (12 month), normal and vasopressin-deficient Brattleboro rats. To receive a comprehensive picture about their memory we examined their social discrimination, object discrimination and conditioned learning abilities (shuttle box). Amyloid precursor protein (APP), mitogen-activated protein kinase 1 (MAPK1), β-actin and tryptophan 2,3-dioxygenase 2 (TDO2) mRNA levels was measured by quantitative PCR.There was no difference between the memory of adult and aged groups. The vasopressin-deficient rats at both ages showed a weaker performance in the course of social and object discrimination tests and a higher escape failure during the shuttle box experiment. The brain marker mRNAs of the elder animals were higher than the levels of the adults, but the absence of vasopressin had no influence on them.Thus, the one year old rats showed elevated levels of AD-related markers, but memory deficits were observable only in vasopressin deficient animals. Vasopressin does not seem to have pathogenic role in AD. Changes in the studied markers might predict later symptoms, although further studies are required for confirmation.

Original languageEnglish
Pages (from-to)6-13
Number of pages8
JournalBrain Research Bulletin
Volume100
DOIs
Publication statusPublished - 2014

Fingerprint

Brattleboro Rats
Vasopressins
Alzheimer Disease
Social Discrimination
Tryptophan Oxygenase
Discrimination Learning
Messenger RNA
Aptitude
Amyloid beta-Protein Precursor
Mitogen-Activated Protein Kinase 1
Memory Disorders
Brain
Dementia
Actins
Polymerase Chain Reaction

Keywords

  • APP
  • MAPK1
  • Object and social discrimination
  • Shuttle box
  • TDO2
  • β-Actin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Increase in Alzheimer's related markers preceeds memory disturbances : Studies in vasopressin-deficient Brattleboro rat. / Varga, János; Klausz, Barbara; Domokos, Ágnes; Kálmán, Sára; Pákáski, M.; Szucs, Szabina; Garab, Dénes; Zvara, A.; Puskás, L.; Kálmán, J.; Tímár, Júlia; Bagdy, G.; Zelena, D.

In: Brain Research Bulletin, Vol. 100, 2014, p. 6-13.

Research output: Contribution to journalArticle

Varga, János ; Klausz, Barbara ; Domokos, Ágnes ; Kálmán, Sára ; Pákáski, M. ; Szucs, Szabina ; Garab, Dénes ; Zvara, A. ; Puskás, L. ; Kálmán, J. ; Tímár, Júlia ; Bagdy, G. ; Zelena, D. / Increase in Alzheimer's related markers preceeds memory disturbances : Studies in vasopressin-deficient Brattleboro rat. In: Brain Research Bulletin. 2014 ; Vol. 100. pp. 6-13.
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abstract = "Alzheimer's disease (AD) is the most common form of dementia in the elderly. For more effective therapy early diagnostic markers could be beneficial. Therefore we compared one year old rats with adults and examined if changes in possible brain markers of AD preceeded memory decline. We also tested if vasopressin-deficient animals were useful model of AD as vasopressin has well known positive effect on memory and AD patient has decreased vasopressin production.We compared adult (3 month) and old (12 month), normal and vasopressin-deficient Brattleboro rats. To receive a comprehensive picture about their memory we examined their social discrimination, object discrimination and conditioned learning abilities (shuttle box). Amyloid precursor protein (APP), mitogen-activated protein kinase 1 (MAPK1), β-actin and tryptophan 2,3-dioxygenase 2 (TDO2) mRNA levels was measured by quantitative PCR.There was no difference between the memory of adult and aged groups. The vasopressin-deficient rats at both ages showed a weaker performance in the course of social and object discrimination tests and a higher escape failure during the shuttle box experiment. The brain marker mRNAs of the elder animals were higher than the levels of the adults, but the absence of vasopressin had no influence on them.Thus, the one year old rats showed elevated levels of AD-related markers, but memory deficits were observable only in vasopressin deficient animals. Vasopressin does not seem to have pathogenic role in AD. Changes in the studied markers might predict later symptoms, although further studies are required for confirmation.",
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AU - Szucs, Szabina

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AU - Bagdy, G.

AU - Zelena, D.

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