Incorporation of spin-labelled amino acids into proteins

Christian F W Becker, Kester Lausecker, Mária Balog, T. Kalai, K. Hideg, Heinz Jürgen Steinhoff, Martin Engelhard

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The elucidation of structure and function of proteins and membrane proteins by EPR spectroscopy has become increasingly important in recent years as technological advances have been made in the design of spectrometers and in the chemistry of the nitroxide group. These new developments have increased the demand for tailor-made amino acids carrying a spin label on the one hand and for reliable methods for their incorporation into proteins on the other. Here we describe methods for site-specific spin labelling of proteins. It is shown that a combination of recombinant synthesis of proteins with chemically produced peptides (expressed protein ligation) allows the preparation of site-specifically spin-labelled proteins.

Original languageEnglish
JournalMagnetic Resonance in Chemistry
Volume43
Issue numberSPEC. ISS.
DOIs
Publication statusPublished - Nov 2005

Fingerprint

amino acids
Amino acids
proteins
Proteins
Amino Acids
Spin Labels
Labeling
Paramagnetic resonance
Spectrometers
Membrane Proteins
Spectroscopy
Peptides
marking
peptides
Labels
spectrometers
chemistry
membranes
Membranes
preparation

Keywords

  • Chemical synthesis of proteins
  • EPR spectroscopy
  • Protein ligation
  • Site-directed spin labelling

ASJC Scopus subject areas

  • Chemistry(all)
  • Physical and Theoretical Chemistry
  • Spectroscopy

Cite this

Becker, C. F. W., Lausecker, K., Balog, M., Kalai, T., Hideg, K., Steinhoff, H. J., & Engelhard, M. (2005). Incorporation of spin-labelled amino acids into proteins. Magnetic Resonance in Chemistry, 43(SPEC. ISS.). https://doi.org/10.1002/mrc.1688

Incorporation of spin-labelled amino acids into proteins. / Becker, Christian F W; Lausecker, Kester; Balog, Mária; Kalai, T.; Hideg, K.; Steinhoff, Heinz Jürgen; Engelhard, Martin.

In: Magnetic Resonance in Chemistry, Vol. 43, No. SPEC. ISS., 11.2005.

Research output: Contribution to journalArticle

Becker, CFW, Lausecker, K, Balog, M, Kalai, T, Hideg, K, Steinhoff, HJ & Engelhard, M 2005, 'Incorporation of spin-labelled amino acids into proteins', Magnetic Resonance in Chemistry, vol. 43, no. SPEC. ISS.. https://doi.org/10.1002/mrc.1688
Becker, Christian F W ; Lausecker, Kester ; Balog, Mária ; Kalai, T. ; Hideg, K. ; Steinhoff, Heinz Jürgen ; Engelhard, Martin. / Incorporation of spin-labelled amino acids into proteins. In: Magnetic Resonance in Chemistry. 2005 ; Vol. 43, No. SPEC. ISS.
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