Incorporation of pantothenate into citrate lyase by a pantothenateless mutant of Klebsiella pneumoniae

M. Singh, W. B. Dempsey, P. A. Srere

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A pantothenate requiring mutant of K. pneumoniae was isolated. The mutant showed an absolute dependence on pantothenate for growth. When grown in the presence of [14C]pantothenate, the mutant incorporated [14C]pantothenate into citrate lyase (3.4 mol/mol of enzyme). Analysis of a double labeled enzyme ([14C]pantothenate and [3H]acetate) by gel electrophoresis in sodium dodecyl sulfate showed that both 3H and 14C were associated solely with the smallest subunit, the acyl carrier protein of citrate lyase.

Original languageEnglish
Pages (from-to)686-692
Number of pages7
JournalJournal of bacteriology
Volume124
Issue number2
DOIs
Publication statusPublished - 1975

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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