Inconsistencies in the red blood cell membrane proteome analysis

Generation of a database for research and diagnostic applications

T. Hegedűs, Pururawa Mayank Chaubey, György Várady, Edit Szabó, Hajnalka Sarankó, Lia Hofstetter, Bernd Roschitzki, Bruno Stieger, B. Sarkadi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Based on recent results, the determination of the easily accessible red blood cell (RBC) membrane proteins may provide new diagnostic possibilities for assessing mutations, polymorphisms or regulatory alterations in diseases. However, the analysis of the current mass spectrometry-based proteomics datasets and other major databases indicates inconsistencies - the results show large scattering and only a limited overlap for the identified RBC membrane proteins. Here, we applied membrane-specific proteomics studies in human RBC, compared these results with the data in the literature, and generated a comprehensive and expandable database using all available data sources. The integrated web database now refers to proteomic, genetic and medical databases as well, and contains an unexpected large number of validated membrane proteins previously thought to be specific for other tissues and/or related to major human diseases. Since the determination of protein expression in RBC provides a method to indicate pathological alterations, our database should facilitate the development of RBC membrane biomarker platforms and provide a unique resource to aid related further research and diagnostics.

Original languageEnglish
Article numberbav056
JournalDatabase : the journal of biological databases and curation
Volume2015
DOIs
Publication statusPublished - 2015

Fingerprint

Proteome
Cell membranes
proteome
cell membranes
Blood
erythrocytes
Erythrocytes
Cell Membrane
Databases
Proteins
membrane proteins
Proteomics
proteomics
Membrane Proteins
Research
Membranes
Genetic Databases
Information Storage and Retrieval
Biomarkers
Polymorphism

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Information Systems

Cite this

Inconsistencies in the red blood cell membrane proteome analysis : Generation of a database for research and diagnostic applications. / Hegedűs, T.; Chaubey, Pururawa Mayank; Várady, György; Szabó, Edit; Sarankó, Hajnalka; Hofstetter, Lia; Roschitzki, Bernd; Stieger, Bruno; Sarkadi, B.

In: Database : the journal of biological databases and curation, Vol. 2015, bav056, 2015.

Research output: Contribution to journalArticle

Hegedűs, T. ; Chaubey, Pururawa Mayank ; Várady, György ; Szabó, Edit ; Sarankó, Hajnalka ; Hofstetter, Lia ; Roschitzki, Bernd ; Stieger, Bruno ; Sarkadi, B. / Inconsistencies in the red blood cell membrane proteome analysis : Generation of a database for research and diagnostic applications. In: Database : the journal of biological databases and curation. 2015 ; Vol. 2015.
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