Inactivation of phages T7 and phi X174 induced by the radiomimetics N nitroso N methylurea (NMU) and N acetylethylenimine (AEI) has been studied. In semilogarithmic scale and the kinetic curves of phage inactivation were steplike in character. This was connected with the occurrence of several processes in the system. A possible kinetic model has been proposed for the process NMU induced phage inactivation. The model is applicable to initial phage inactivation only. From the model a kinetic curve parameter, rho can be determined which characterizes the radiomimetic effect on different phages and determines the changes in the repair system after caffeine treatment. NMU exerted a post inactivation effect on the phage T7. Also the NMU induced phage T7 post inactivation kinetic curves were of steplike character. They can be described as a sum of two exponential processes of different rate constant. The rate constant for the first stage of the post inactivation process was close to the depurinization rate constant found in the literature.
|Number of pages||8|
|Journal||Acta Biochimica et Biophysica Academiae Scientiarum Hungaricae|
|Publication status||Published - Dec 1 1975|
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