Inactivation of α- and β-thrombin by antithrombin-III, α2-macroglobulin and α1-proteinase inhibitor

R. Machovich, A. Borsodi, G. Blasko, S. A. Orakzai

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Abstract

Inactivation of α- and β-thrombin by α2-macroglobulin, by α1-proteinase inhibitor and by antithrombin-III and heparin was studied. The amount of α- and β-thrombin inactivated by antithrombin-III was proportional to the concentration of the inhibitor, but the inactivation rates of the two forms of thrombin were different. Heparin facilitated complex-formation between α-thrombin and antithrombin-III, whereas inactivation of β-thrombin by antithrombin was only slightly influenced, even at a heparin concentration two orders of magnitude higher. α2-Macroglobulin inhibited both α- and β-thrombin activity similarly, i.e. the amount of α- and β-thrombin inactivated as well as the rates of their inhibition were the same. α1-Proteinase inhibitor also formed a complex with α- and β-thrombin, similarly to antithrombin-III, although the inactivation of the enzyme needed high inhibitor concentration and long incubation time. These results suggest that the inactivation of β-thrombin, if it occurs in the plasma, is also controlled by plasma inhibitors.

Original languageEnglish
Pages (from-to)393-398
Number of pages6
JournalBiochemical Journal
Volume167
Issue number2
Publication statusPublished - 1977

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Macroglobulins
Antithrombin III
Thrombin
Peptide Hydrolases
Heparin
Plasmas
Antithrombins

ASJC Scopus subject areas

  • Biochemistry

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Inactivation of α- and β-thrombin by antithrombin-III, α2-macroglobulin and α1-proteinase inhibitor. / Machovich, R.; Borsodi, A.; Blasko, G.; Orakzai, S. A.

In: Biochemical Journal, Vol. 167, No. 2, 1977, p. 393-398.

Research output: Contribution to journalArticle

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