Inactivation kinetics of horseradish peroxidase microinjected into hepatocytes from mice of various ages

Akihito Ishigami, Sataro Goto

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We have studied degradation of proteins in liver parenchymal cells in culture obtained from mice of various ages. Horseradish peroxidase (HRP) was microinjected into the cells by the hypertonic-hypotonic method of Okada and Rechsteiner (Cell, 29 (1982) 33-41). Rate of inactivation of HRP was examined biochemically. Average functional half-lives of the enzyme were 50 h, 53 h and 75 h in the cells of young (3-8 months old), middle-aged (18-20 months old) and old (28-30 months old) mice, respectively. Thus, half-life of the inactivation of the enzyme in "old" cells was about 50% longer than that in "young" or "middle-aged" cells, suggesting that rate of protein degradation is lowered in senescent animals.

Original languageEnglish
Pages (from-to)125-133
Number of pages9
JournalMechanisms of Ageing and Development
Volume46
Issue number1-3
DOIs
Publication statusPublished - 1988

Keywords

  • Aging
  • Horseradish peroxidase
  • Microinjection
  • Mouse hepatocyte
  • Protein turnover

ASJC Scopus subject areas

  • Ageing
  • Developmental Biology

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