In vivo study of targeted nanomedicine delivery into Langerhans cells by multiphoton laser scanning microscopy

Attila Kolonics, Zsolt Csiszovszki, Eniko R. Toke, Orsolya Lorincz, Dóra Haluszka, Róbert Szipocs

Research output: Contribution to journalLetter

9 Citations (Scopus)

Abstract

Epidermal Langerhans cells (LCs) function as professional antigen-presenting cells of the skin. We investigated the LC-targeting properties of a special mannose-moiety-coated pathogen-like synthetic nanomedicine DermaVir (DV), which is capable to express antigens to induce immune responses and kill HIV-infected cells. Our aim was to use multiphoton laser microscopy (MLM) in vivo in order to visualize the uptake of Alexa-labelled DV (AF546-DV) by LCs. Knock-in mice expressing enhanced green fluorescent protein (eGFP) under the control of the langerin gene (CD207) were used to visualize LCs. After 1 h, AF546-DV penetrated the epidermis and entered the eGFP-LCs. The AF546-DV signal was equally distributed inside the LCs. After 9 h, we observed AF546-DV signal accumulation that occurred mainly at the cell body. We demonstrated in live animals that LCs picked up and accumulated the nanoparticles in the cell body.

Original languageEnglish
Pages (from-to)596-605
Number of pages10
JournalExperimental Dermatology
Volume23
Issue number8
DOIs
Publication statusPublished - Aug 2014

Keywords

  • EGFP-Langerin knock-in mice
  • Langerhans cells
  • Multiphoton laser microscopy
  • Nanomedicine formulation
  • in vivo

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

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