Commercial geese (Gray Landes) were examined by means of high-resolution spiral computer tomography in order to follow their liver development in vivo. Three ganders were scanned six times before, within and after a force-feeding period. 3D images of the liver were reconstructed from the 2D transverse slices with segmentation and rendering methods. The changes in the external surface, the volume of the liver and also the characteristic Hounsfield values were determined. The livers of another 70 ganders were examined by CT, then by direct chemical analysis (days 0, 13, 18, 19, 20 and 21 of force-feeding). To estimate the chemical composition of the tissue, prediction equations were developed based on the pixel frequency distributions. With partial least squares (PLS) regression, the ether extract and crude protein content could be estimated with R2 = 0.97 and R2 = 0.96 accuracy, respectively. Data analysis was complemented with serial blood serum measurements characteristic of liver steatosis. The method applied may be a unique possibility to study the real geometrical relations of liver development and also to describe the qualitative changes of tissue composition during the force-feeding period in vivo, with special regards to selection purposes.
ASJC Scopus subject areas
- Animal Science and Zoology