In vivo studies on Aujeszky's disease virus mutants.

Z. Boldogkői, I. Medveczky, R. Glávits, A. Braun, I. Fodor

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We report the construction and in vivo analysis of three recombinant Aujeszky's disease virus (ADV) strains containing mutations at three different loci of the genome. Mutant vE16lac was generated by deleting of 2976 bp DNA fragment which covers 1851 bp of the right arm of UL component, the UL-US junction, the "a" element of the internal repeat (IR) region and a putative LAT promoter. Mutant vRRlac was generated by deletion of a 1805 bp fragment from the coding region of the large and small subunits of ribonucleotide reductase gene (rr). The third mutant, vTKlac, was constructed using insertional mutagenesis of the thymidine kinase gene (tk). In the constructed mutants a lacZ gene expression cassette was either inserted into the target gene (vTKlac) or replaced the deleted DNA segment (vE16lac, vRRlac). Constructed recombinant viruses were analyzed by infecting pigs and monitoring the virus excretion from nasal fluid and disease symptoms. Tissue specimens were collected for virus isolation and pathological examination. Strains vTKlac and vRRlac retained the ability to establish an infection, but showed reduced replication efficiency in the respiratory tract and were unable to attack the central nervous system (CNS) of pigs. Thus, both deletions induce significant attenuation of the virus measured by decrease of virulence in infected pigs. Strain vE16lac showed disease symptoms similar to that of wild type and could be detected in the CNS of pigs.

Original languageEnglish
Pages (from-to)307-318
Number of pages12
JournalActa Microbiologica et Immunologica Hungarica
Volume43
Issue number4
Publication statusPublished - 1996

Fingerprint

Suid Herpesvirus 1
Swine
Viruses
Central Nervous System
Nose Diseases
Genes
Ribonucleotide Reductases
Lac Operon
Thymidine Kinase
Insertional Mutagenesis
DNA
Respiratory System
Virulence
Genome
Gene Expression
Mutation
Infection

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Medicine(all)
  • Microbiology

Cite this

Boldogkői, Z., Medveczky, I., Glávits, R., Braun, A., & Fodor, I. (1996). In vivo studies on Aujeszky's disease virus mutants. Acta Microbiologica et Immunologica Hungarica, 43(4), 307-318.

In vivo studies on Aujeszky's disease virus mutants. / Boldogkői, Z.; Medveczky, I.; Glávits, R.; Braun, A.; Fodor, I.

In: Acta Microbiologica et Immunologica Hungarica, Vol. 43, No. 4, 1996, p. 307-318.

Research output: Contribution to journalArticle

Boldogkői, Z, Medveczky, I, Glávits, R, Braun, A & Fodor, I 1996, 'In vivo studies on Aujeszky's disease virus mutants.', Acta Microbiologica et Immunologica Hungarica, vol. 43, no. 4, pp. 307-318.
Boldogkői, Z. ; Medveczky, I. ; Glávits, R. ; Braun, A. ; Fodor, I. / In vivo studies on Aujeszky's disease virus mutants. In: Acta Microbiologica et Immunologica Hungarica. 1996 ; Vol. 43, No. 4. pp. 307-318.
@article{a7662820c98146f88e3c33cc7741eba0,
title = "In vivo studies on Aujeszky's disease virus mutants.",
abstract = "We report the construction and in vivo analysis of three recombinant Aujeszky's disease virus (ADV) strains containing mutations at three different loci of the genome. Mutant vE16lac was generated by deleting of 2976 bp DNA fragment which covers 1851 bp of the right arm of UL component, the UL-US junction, the {"}a{"} element of the internal repeat (IR) region and a putative LAT promoter. Mutant vRRlac was generated by deletion of a 1805 bp fragment from the coding region of the large and small subunits of ribonucleotide reductase gene (rr). The third mutant, vTKlac, was constructed using insertional mutagenesis of the thymidine kinase gene (tk). In the constructed mutants a lacZ gene expression cassette was either inserted into the target gene (vTKlac) or replaced the deleted DNA segment (vE16lac, vRRlac). Constructed recombinant viruses were analyzed by infecting pigs and monitoring the virus excretion from nasal fluid and disease symptoms. Tissue specimens were collected for virus isolation and pathological examination. Strains vTKlac and vRRlac retained the ability to establish an infection, but showed reduced replication efficiency in the respiratory tract and were unable to attack the central nervous system (CNS) of pigs. Thus, both deletions induce significant attenuation of the virus measured by decrease of virulence in infected pigs. Strain vE16lac showed disease symptoms similar to that of wild type and could be detected in the CNS of pigs.",
author = "Z. Boldogkői and I. Medveczky and R. Gl{\'a}vits and A. Braun and I. Fodor",
year = "1996",
language = "English",
volume = "43",
pages = "307--318",
journal = "Acta Microbiologica et Immunologica Hungarica",
issn = "1217-8950",
publisher = "Akademiai Kiado",
number = "4",

}

TY - JOUR

T1 - In vivo studies on Aujeszky's disease virus mutants.

AU - Boldogkői, Z.

AU - Medveczky, I.

AU - Glávits, R.

AU - Braun, A.

AU - Fodor, I.

PY - 1996

Y1 - 1996

N2 - We report the construction and in vivo analysis of three recombinant Aujeszky's disease virus (ADV) strains containing mutations at three different loci of the genome. Mutant vE16lac was generated by deleting of 2976 bp DNA fragment which covers 1851 bp of the right arm of UL component, the UL-US junction, the "a" element of the internal repeat (IR) region and a putative LAT promoter. Mutant vRRlac was generated by deletion of a 1805 bp fragment from the coding region of the large and small subunits of ribonucleotide reductase gene (rr). The third mutant, vTKlac, was constructed using insertional mutagenesis of the thymidine kinase gene (tk). In the constructed mutants a lacZ gene expression cassette was either inserted into the target gene (vTKlac) or replaced the deleted DNA segment (vE16lac, vRRlac). Constructed recombinant viruses were analyzed by infecting pigs and monitoring the virus excretion from nasal fluid and disease symptoms. Tissue specimens were collected for virus isolation and pathological examination. Strains vTKlac and vRRlac retained the ability to establish an infection, but showed reduced replication efficiency in the respiratory tract and were unable to attack the central nervous system (CNS) of pigs. Thus, both deletions induce significant attenuation of the virus measured by decrease of virulence in infected pigs. Strain vE16lac showed disease symptoms similar to that of wild type and could be detected in the CNS of pigs.

AB - We report the construction and in vivo analysis of three recombinant Aujeszky's disease virus (ADV) strains containing mutations at three different loci of the genome. Mutant vE16lac was generated by deleting of 2976 bp DNA fragment which covers 1851 bp of the right arm of UL component, the UL-US junction, the "a" element of the internal repeat (IR) region and a putative LAT promoter. Mutant vRRlac was generated by deletion of a 1805 bp fragment from the coding region of the large and small subunits of ribonucleotide reductase gene (rr). The third mutant, vTKlac, was constructed using insertional mutagenesis of the thymidine kinase gene (tk). In the constructed mutants a lacZ gene expression cassette was either inserted into the target gene (vTKlac) or replaced the deleted DNA segment (vE16lac, vRRlac). Constructed recombinant viruses were analyzed by infecting pigs and monitoring the virus excretion from nasal fluid and disease symptoms. Tissue specimens were collected for virus isolation and pathological examination. Strains vTKlac and vRRlac retained the ability to establish an infection, but showed reduced replication efficiency in the respiratory tract and were unable to attack the central nervous system (CNS) of pigs. Thus, both deletions induce significant attenuation of the virus measured by decrease of virulence in infected pigs. Strain vE16lac showed disease symptoms similar to that of wild type and could be detected in the CNS of pigs.

UR - http://www.scopus.com/inward/record.url?scp=0030341855&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030341855&partnerID=8YFLogxK

M3 - Article

C2 - 9147722

AN - SCOPUS:0030341855

VL - 43

SP - 307

EP - 318

JO - Acta Microbiologica et Immunologica Hungarica

JF - Acta Microbiologica et Immunologica Hungarica

SN - 1217-8950

IS - 4

ER -