In vivo Photoacoustic Spectra of Herbicide-Treated Bean Leaves

Z. Szigeti, Eckehard M. Nagel, Claus Buschmann, Hartmut K. Lichtenthaler

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Photoacoustic spectra of control leaves and herbicide-treated bean leaves (Phaseolus vulgaris L. cv. Fori GS) were taken with a modulation frequency of 22 Hz. The excised leaves were floated in water (water control) or in a 10−4 M herbicide solution. The action of PS II inhibitors of the urea type (diuron) and of the phenol type (3-bromo-4-hydroxy-5-nitro-benzonitrile) were compared to that of the non-selective herbicide, paraquat. After 5 h of incubation the paraquat-treated leaves were characterized by a reduced carotenoid content, lower chlorophyll fluorescence yield and a lower variable portion in the fluorescence kinetics. After treatment with the PS II inhibitors, diuron and benzonitrile, the pigment content was not changed; the chlorophyll fluorescence yield was increased, whereas the variable fluorescence was decreased. The three types of chemicals induced a decrease of the in vivo photoacoustic signal in the entire spectral range from 380 to 720 nm, which was paralleled by a decline in photosynthetic function as seen from low Rfd-values. This decrease in the photoacoustic signal was more pronounced in the red than in the blue spectral region. The photoacoustic signal is partially determined by a pulsed, photosynthetic oxygen evolution. This is seen from the addition of continuous white light, which leads to a similar decrease of the photoacoustic signal as the herbicide treatment since the homogeneously produced oxygen no longer induces a photoacoustic signal. The results are discussed in view of the application of photoacoustic spectroscopy for the in vivo investigation of physiological effects of photosynthetic herbicides.

Original languageEnglish
Pages (from-to)104-109
Number of pages6
JournalJournal of Plant Physiology
Volume134
Issue number1
DOIs
Publication statusPublished - Jan 1 1989

Fingerprint

Herbicides
beans
herbicides
Fluorescence
fluorescence
Diuron
diuron
Paraquat
paraquat
leaves
Chlorophyll
photoacoustic spectroscopy
Oxygen
chlorophyll
Phaseolus
Water
white light
Carotenoids
Phenol
phenol

Keywords

  • 3-bromo-4-hydroxy-5-nitro-benzonitrile
  • benzonitrile
  • BN
  • diuron
  • F690 and F735
  • F690/F735
  • fd
  • fluorescence decrease
  • fluorescence decrease ratio (= fd/fs)
  • fluorescence decrease ratio at the given wavelengths regions
  • fluorescence signal at the two fluorescence maxima near 690 and 735 nm, respectively
  • fs
  • herbicide effects
  • PA675/PA475
  • paraquat
  • Phaseolus vulgaris
  • photoacoustic spectroscopy
  • ratio of the fluorescence at the given wavelengths
  • ratio of the photoacoustic signal at the given wavelengths
  • Rfd
  • Rfd690 and Rfd730
  • steady state fluorescence

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

In vivo Photoacoustic Spectra of Herbicide-Treated Bean Leaves. / Szigeti, Z.; Nagel, Eckehard M.; Buschmann, Claus; Lichtenthaler, Hartmut K.

In: Journal of Plant Physiology, Vol. 134, No. 1, 01.01.1989, p. 104-109.

Research output: Contribution to journalArticle

Szigeti, Z. ; Nagel, Eckehard M. ; Buschmann, Claus ; Lichtenthaler, Hartmut K. / In vivo Photoacoustic Spectra of Herbicide-Treated Bean Leaves. In: Journal of Plant Physiology. 1989 ; Vol. 134, No. 1. pp. 104-109.
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