In vivo measurement of the disaggregatory action of prostacyclin. A methological study

Ferenc Herman, Pál Hadhazy, Kálman Magyar

Research output: Contribution to journalArticle

Abstract

The disaggregating effect of PGI2 was measured in a modified in vivo model described by Hornstra (1). The arterial blood of mongrel dogs was directed through an extracorporeal filter by a roller pump, and the pressure proximal to the filter was measured. The filter became spontaneously occluded mostly by aggregates of platelets within a few minutes, and as a result the pressure before the filter increased continuously.PGI2, administered either before the filter or intravenously, produced dose-related reduction in filtration pressure. Results obtained in control experiments revealed that 1/ the rate of platelet aggregation was reproducible; 2/ neither the count of platelets in the circulating blood nor the in vitro sensitivity of platelets to ADP and PGI2 changed significantly in the course of an experiment. The technique described seems to be useful to determine the disaggregatory potency of prostacyclin and other substances.

Original languageEnglish
Pages (from-to)575-585
Number of pages11
JournalThrombosis research
Volume44
Issue number5
DOIs
Publication statusPublished - Dec 1 1986

Keywords

  • Platelet aggregation
  • platelet disaggregation
  • prostacyclin
  • screen filtration pressure

ASJC Scopus subject areas

  • Hematology

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