In vivo inhibition of polyamine oxidase by a spermine analogue, MDL-72527, in tomato exposed to sublethal and lethal salt stress

Zoltán Takács, Péter Poór, Ágnes Szepesi, I. Tari

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The spermine analogue N1,N4-bis-(2,3-butadienyl)-1,4-butanediamine (MDL-72527), an effective inhibitor of polyamine oxidases (PAOs), triggers a systemic response in tomato (Solanum lycopersicum L.) exposed to sublethal (100mM) and lethal (250mM) NaCl concentrations. The accumulation of free polyamines (PAs), the terminal oxidation of PAs by diamine oxidases (DAOs) and PAOs, and the production of H2O2 by PA oxidases depends on the intensity of salt stress. Spermidine and spermine content increased significantly under sublethal salt concentrations, but remained low under lethal salt stress. Along with increased expression of the selected SlDAO1 and SlPAO1 genes in the leaves and roots, respectively, DAO and PAO activities and their product, H2O2, increased and initiated cell death by irreversible loss of electrolytes at 250mM NaCl. MDL-72527 significantly increased spermine, spermidine and/or putrescine contents as a result of reduced activity of PA oxidases; furthermore, it inhibited H2O2 and NO production during salt treatment. These results indicate that PAO contributed to H2O2 and NO production under salt stress, and the terminal activities of DAO and PAO play a role in cell death induction at 250mM NaCl. However, the inhibition of PAO by MDL-72527 does not increase the salt tolerance of plants, since electrolyte leakage increased significantly in the presence of the inhibitor.

Original languageEnglish
Pages (from-to)480-492
Number of pages13
JournalFunctional Plant Biology
Volume44
Issue number5
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

spermine
polyamines
lethal genes
salt stress
tomatoes
spermidine
electrolytes
cell death
Solanum lycopersicum
putrescine
salt tolerance
salt concentration
oxidation
salts

Keywords

  • diamine oxidase
  • electrolyte leakage
  • nitric oxide
  • programmed cell death.
  • reactive oxygen species

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

In vivo inhibition of polyamine oxidase by a spermine analogue, MDL-72527, in tomato exposed to sublethal and lethal salt stress. / Takács, Zoltán; Poór, Péter; Szepesi, Ágnes; Tari, I.

In: Functional Plant Biology, Vol. 44, No. 5, 01.01.2017, p. 480-492.

Research output: Contribution to journalArticle

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