In vivo effects of DL-α-difluoromethylornithine on the polyamine and nucleotide phosphate metabolism in P388/S leukemia cells

T. Kremmer, M. Boldizsár, L. Holczinger

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In vivo effects of DL-α-difluoromethylomithine (DFMO) on the metabolism of polyamines and nucleotide phosphates were monitored in P388/S leukemia cells grown intra-peritoneally in BDF1 inbred male mice. Inhibiting the ornithine decarboxylase (ODC) activity DFMO depleted putrescine and spermidine to 30-50 and 50-60%, respectively, and increased spermine to 25-60% compared with the controls, when given as 2% solution in drinking water of the tumor-bearing animals. DFMO treatment caused a parallel 56% elevation of total nucleotide content in tumor cells with distinct and significant increase of some nucleotide phosphates. The most pronounced alterations were shown in the intracellular UTP (202%), CTP (103%), ADP (92%) and ATP (71 %) concentrations. Changes in polyamine and nucleotide phosphate metabolisms were dependent on tumor progression. A possible explanation of the metabolic events induced by DFMO is discussed.

Original languageEnglish
Pages (from-to)8-15
Number of pages8
JournalPathobiology
Volume54
Issue number1
DOIs
Publication statusPublished - Jan 1 1986

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Keywords

  • DL-α-Difluoromethylornithine
  • Nucleotide phosphates
  • P388 leukemia cells
  • Polyamine depletion

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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