In vivo effects of cyclophosphamide on oncogene and suppressor gene expression in a 'follow up' study

István Ember, István Kiss

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cyclophosphamide is a chemotherapeutical drug, with proved carcinogenic side effects. Our previous experiments showed its effect on the expression of certain oncogenes and tumor suppressor genes. In order to further explore the effect of cyclophosphamide at the gene level an in vivo mouse model was developed. We compared the effects of cyclophosphamide with that of cyclosporine, which is a non genotoxic human carcinogenic chemical. After different time periods of intraperitoneal cyclophosphamide or cyclosporine injection, RNA was isolated from whole organs of experimental animals, and expression of onco/suppressor genes was determined by slot blot hybridisation. In our model we have proved that gene expression changes caused by cyclophosphamide can be detected even six months after treatment without tumor development. Our results support the hypothesis that gene expression investigations could be useful biomarkers in monitoring the effects of environmental carcinogenic compounds.

Original languageEnglish
Pages (from-to)3593-3597
Number of pages5
JournalAnticancer research
Volume17
Issue number5 A
Publication statusPublished - Sep 1 1997

Keywords

  • Cyclophosphamide
  • Cyclosporine
  • Gene expression
  • Oncogenes
  • Secondary tumors

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'In vivo effects of cyclophosphamide on oncogene and suppressor gene expression in a 'follow up' study'. Together they form a unique fingerprint.

  • Cite this